# High-accuracy, long-range sequencing for HIV-1 genotyping

> **NIH NIH R21** · OHIO STATE UNIVERSITY · 2020 · $234,000

## Abstract

PROJECT SUMMARY / ABSTRACT
Innovations in sequencing technologies over the past decade have been critical driving forces behind the
ongoing revolution in medicine and the life sciences. The Nanopore sequencing platform (Oxford Nanopore
Technologies) is a newer, so-called third generation sequencing technology with several futuristic features,
including a tremendously long-read length capacity, real-time data output, and pocket-size mobility (thanks to
the MinION sequencer). A critical barrier to broader applications of this emerging technology has been its
disappointingly high sequencing error rates. The overall goal of this study is to develop a novel barcoding
technology with which to radically improve the accuracy of single-molecule, target DNA sequencing for the
Nanopore sequencing platform. Long-range, high-accuracy sequencing will be essential in delineating the
heterogeneity of cellular or viral populations in diseases such as cancer or viral infection. In this proposal, we
will focus on the genotyping of HIV-1 as a proof of principle. HIV-1 is an excellent model system because of its
relatively small genome (approximately 9 Kbp), the high degree of intra-patient genetic diversity, and the
availability of sufficient NGS and Sanger sequence data for the purpose of comparison. We will develop and
establish standard experimental procedures for generating a new-generation barcode library and for using this
library to perform sequencing error correction processes (Aims 1 and 2); We will then test the utility of our
method using laboratory strains of HIV-1 (Aims 3).

## Key facts

- **NIH application ID:** 9933970
- **Project number:** 5R21HG010108-03
- **Recipient organization:** OHIO STATE UNIVERSITY
- **Principal Investigator:** Sanggu Kim
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $234,000
- **Award type:** 5
- **Project period:** 2018-08-01 → 2022-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9933970

## Citation

> US National Institutes of Health, RePORTER application 9933970, High-accuracy, long-range sequencing for HIV-1 genotyping (5R21HG010108-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9933970. Licensed CC0.

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