# Curriculum: principles of cryo-EM structure determination

> **NIH NIH R25** · YALE UNIVERSITY · 2020 · $124,200

## Abstract

The cryo-EM determination of atomic structures by single-particle methods represents a revolution in
structural biology. The method relies on the astonishing ability of numerical computations to yield high-
resolution density maps from collections of extremely noisy “particle” images. This ability however
carries with it a real danger of spurious results emerging from the black box of image-processing and
reconstruction software. We propose to develop an educational curriculum including video, software
and an e-book that provides a rigorous treatment of the mathematical foundations of cryo-EM image
processing and reconstruction. The goal of our instructional efforts is to ground students and
researchers in the understanding of the principles and best-practice methods in the computational
processing that leads to molecular structures.

## Key facts

- **NIH application ID:** 9934207
- **Project number:** 5R25EY029125-03
- **Recipient organization:** YALE UNIVERSITY
- **Principal Investigator:** FREDERICK J SIGWORTH
- **Activity code:** R25 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $124,200
- **Award type:** 5
- **Project period:** 2018-05-01 → 2022-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9934207

## Citation

> US National Institutes of Health, RePORTER application 9934207, Curriculum: principles of cryo-EM structure determination (5R25EY029125-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9934207. Licensed CC0.

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