# Core B: Proteomics and lipoprotein characterization core

> **NIH NIH P01** · UNIVERSITY OF WASHINGTON · 2020 · $177,776

## Abstract

The proteomics and lipoprotein characterization core (Core B) will provide the powerful tools of modern
mass spectrometry and complex data set analysis to Program Project Grant (PPG) investigators. The Core will
also provide lipoprotein isolation, and measurements of lipoprotein particle concentration and size using
calibrated ion mobility analysis. The Core will permit structural identification and quantitation of lipoproteins
including remnant lipoprotein particles (RLPs), as well as VLDL, LDL and HDL, and measurement of
lipoprotein particle number of these lipoprotein classes using calibrated ion mobility analysis.
Core personnel are proficient in liquid chromatography-electrospray ionization-MS/MS analysis (shotgun
proteomics) of complex biological mixtures of proteins, with a particular emphasis on the lipoprotein proteomes
and on the quantification of their protein cargo using targeted LC-MSMS analysis. The Core Director
established precise methods for quantitation of HDL proteome and applied them to quantification of the HDL
proteome in large translational studies and will apply the same methods to other lipoprotein classes through
Core B. The unique calibrated ion mobility analysis method measures HDL particle number and 5 subspecies
of HDL particles, as well as proatherogenic particles like RLPs, and small dense LDL. We anticipate that all
investigators will take advantage of these capabilities.
In addition to providing instrumental capabilities and bioinformatics tools, Core B staff will provide consultation
and collaboration on the application of mass spectrometry and lipoprotein particle concentration measurements
to the Program Project, and integration and analysis of complex datasets. This will include development of new
analytical methods targeted at achieving the research objectives of the Program’s investigators.
Core B will optimize the efficiency and cost-effectiveness through which these services are provided to PPG
investigators by providing a central laboratory. This avoids the need for individual PPG investigators to
maintain the required instrumentation in their own laboratories, avoids the high cost of commercial mass
spectrometric services, and will ensure consistency between analyses for different project sites (Seattle, St.
Louis, and New York). By centralizing and standardizing procedures, Core B will provide a common set of
analytical tools that will lead to a unified understanding of molecular mechanisms involved in the physiologic
and pathophysiologic processes of diabetic vascular disease.

## Key facts

- **NIH application ID:** 9934531
- **Project number:** 1P01HL151328-01
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Tomas Vaisar
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $177,776
- **Award type:** 1
- **Project period:** 2020-08-15 → 2025-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9934531

## Citation

> US National Institutes of Health, RePORTER application 9934531, Core B: Proteomics and lipoprotein characterization core (1P01HL151328-01). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/9934531. Licensed CC0.

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