# Project 3: Lipolysis regulation and diabetes-impaired regression

> **NIH NIH P01** · UNIVERSITY OF WASHINGTON · 2020 · $505,193

## Abstract

Diabetes is associated with greater cardiovascular disease (CVD), likely due at least in part to increased
circulating levels of atherogenic lipoproteins. The most common dyslipidemia in patients with diabetes,
especially Type 2 diabetes, is hypertriglyceridemia (hyperTG). Moreover, recent human data also implicate
genes associated with hyperTG such as lipoprotein lipase (LPL), apolipoprotein C3 (APOC3), and
angiopoietin-like protein (ANGPTL)3 with CVD. Despite more than 4 decades of investigation, a mechanistic
understanding of the relationship of hyperTG and CVD has not been clearly defined. Project 3 of this Program
Project proposes to develop and use new animal models to assess how hyperTG and diabetes interact to alter
vascular pathobiology. Our studies will focus on changes in arterial LPL using models that increase and
decrease lipolysis within and likely along the arterial wall. Monocyte/macrophages are the only white blood
cells that highly express LPL and they are the primary source of vascular LPL. Using single cell RNA
sequencing, we have found that arterial macrophages have a cluster of high LPL expressing cells and our
studies will uncover how LPL itself and LPL in the presence of hyperTG and diabetes alter cells in this and the
other clusters. This Project has two specific aims. Aim 1 will determine how hyperTG and local lipolysis affect
vascular disease. To do this, we will transplant bone marrow from mice that have an inducible deletion of either
LPL (to reduce lipolysis) or ANGPTL4 (to increased LPL activity) into animals that have either normal or
increased circulating levels of triglyceride (TGs) due to induced global deletion of LPL. Both atherogenesis and
regression will be studied using a novel method we developed to delete and then replenish LDL receptors. In
addition, we will test whether changing the composition of circulating TGs by feeding mice an omega 3 fatty
acid-rich diet, recently shown to reduce CVD risk, will modify the vascular effects of altering macrophage LPL
expression or levels of remnant lipoprotein particles. Aim 2 will test how changes in hyperglycemia/insulin
actions and altered lipolysis products affect atherosclerosis regression, endothelial cell lipid accumulation and
gene expression. We will create models with insulin deficiency and defective insulin sensitivity due to high fat
diets. We were the first to show that defective regression is a robust vascular disease manifestation of
diabetes. These studies will involve extensive interactions with the other projects of this Program and use of
Cores B and C.

## Key facts

- **NIH application ID:** 9934535
- **Project number:** 1P01HL151328-01
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Ira J Goldberg
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $505,193
- **Award type:** 1
- **Project period:** 2020-08-15 → 2025-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9934535

## Citation

> US National Institutes of Health, RePORTER application 9934535, Project 3: Lipolysis regulation and diabetes-impaired regression (1P01HL151328-01). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/9934535. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*