# Cell line Core

> **NIH NIH P01** · UNIV OF NORTH CAROLINA CHAPEL HILL · 2020 · $192,663

## Abstract

PROJECT SUMMARY
This project will investigate mammalian DNA polymerase θ (Pol θ), the defining enzyme for repair of DNA
double-strand breaks by polymerase theta-mediated end joining (TMEJ). This is Core C (“Cell Line
Engineering Core”) which is part of a Program Project titled, “Polymerase theta, genome instability, and
cancer”. Core C will be responsible for providing native and genetically engineered cell lines to support all of
the cell-based assays performed in Projects 1, 2, and 4 of the Program Project.
 The authentication of mammalian cell line reagents is vital for rigor and reproducibility of laboratory
experiments. By establishing and implementing standardized protocols for cell line validation, efficient genome
manipulation, cryopreservation, and dissemination, the Core will ensure quality control of cell line reagents and
minimize the likelihood of erroneous observations and interpretations.
 In Aim 1, “Credentialing of mammalian cell line models utilized in this Program Project”, we will implement
a standardized and comprehensive protocol for authentication and characterization of mammalian cell line
models used across the Program Project.
 In Aim 2, “CRISPR-based cell line genome engineering services for the Program Project”, we will provide
expert services in the design and execution of customized cell line genome engineering tasks in support of
Program Project goals. Cas9 protein utilized in this Aim will be provided by Core B.
 In Aim 3, “Controlled and stable expression of transgenes in mammalian cell lines”, we will utilize
doxycycline-inducible and constitutive transposon vectors for the generation of cell lines that express a variety
of mutagenized and/or tagged transgenes of interest.
 Core C services will be highly coordinated within the Program Project, and will facilitate rigor and
reproducibility, as well as cost-effectiveness, of highly integrated research activities aimed at understanding the
role and targetability of DNA Polymerase q in cancer.

## Key facts

- **NIH application ID:** 9934677
- **Project number:** 1P01CA247773-01
- **Recipient organization:** UNIV OF NORTH CAROLINA CHAPEL HILL
- **Principal Investigator:** Gaorav P. Gupta
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $192,663
- **Award type:** 1
- **Project period:** 2020-07-01 → 2025-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9934677

## Citation

> US National Institutes of Health, RePORTER application 9934677, Cell line Core (1P01CA247773-01). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9934677. Licensed CC0.

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