# Acquisition of BD FACSymphony A3 Lite Flow Cytometer

> **NIH NIH S10** · UNIVERSITY OF WASHINGTON · 2020 · $343,769

## Abstract

PROJECT SUMMARY/ABSTRACT
 This application requests funds to purchase a state-of-the art BD FACS Symphony A3 LITE flow
cytometric analyzer equipped with 5 solid state lasers and capable of detecting 23 unique fluorescent markers.
This instrument will be located at the South Lake Union (SLU) campus of the University of Washington (UW)
accessible to the diverse range of researchers across UW departments and institutes. It will be managed by the
UW Cell Analysis Facility (CAF), the largest flow cytometry shared resource facility at the UW. We have a critical
need for this instrument for two reasons. First, our one aging 18-color LSRII analyzer will be rendered obsolete
due to a lack of support from BD. This is a critical issue as usage on that LSRII alone continues to average
upwards of 145 hours/month, and no equivalent cytometer is available. Second, recent advancements in
instrument and reagent technologies have spurred the development of next generation cytometers that perform
high-content, high-throughput measurements on individual cells, and significantly outperform our existing 14 year
old LSRII system. The A3 will provide us with the capacity to look at more parameters on a single sample,
generating a more granular analysis of diverse cell populations and phenotypic/functional properties, as well as
also permit us to combine previously separated panels into a single composite panel, thus decreasing the total
time to collect the data. The requested BD FACS Symphony A3 is essential to address these needs by providing
timely access to a high parameter cytometer with the advanced technology to further the discovery of biological
networks, definition of disease pathways and development of new cures. This advanced technology will greatly
improve the quality of our researcher’s data and allow them to be more creative and efficient in their approaches.
The impact on biomedical research at the University of Washington will be broad as our user base consists of
105 principle investigators across diverse biomedical disciplines (of which 88% are NIH funded), and continues
to expand. As the University of Washington ranks second among public institutions in receiving NIH funds, it will
be a major benefit to the mission of the NIH to ensure adequate access to current technology for our researchers,
allowing them to utilize their funding efficiently and remain at the forefront of their fields.

## Key facts

- **NIH application ID:** 9934780
- **Project number:** 1S10OD024979-01A1
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Michele C Black
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $343,769
- **Award type:** 1
- **Project period:** 2020-09-01 → 2021-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9934780

## Citation

> US National Institutes of Health, RePORTER application 9934780, Acquisition of BD FACSymphony A3 Lite Flow Cytometer (1S10OD024979-01A1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9934780. Licensed CC0.

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