# PPG Phenotyping

> **NIH NIH P01** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 2020 · $415,844

## Abstract

PROJECT SUMMARY
Core A. Phenotyping
The mission of the Phenotyping Core A is to provide standardized analytical methodologies and services to
assist the Project Investigators in efficiently achieving the Scientific Aims of their respective projects. The Core
will provide targeted phenotyping of both mouse models and human subjects, as well as providing selected
reagents used by the Projects. The Core is organized into 5 Sub-Cores, which will provide the following services:
1) An Atherosclerosis Morphology Sub-Core to provide qualitative and quantitative analyses of mouse
atherosclerosis. 2) Liver Morphology Sub-Core to provide qualitative and quantitative analysis of mouse liver
histology in the context of NASH and fibrosis. 3) Targeted Lipidomic Analysis Sub-Core to provide analyses of
plasma and tissues in the UCSD LIPID MAPS Lipidomic Core. 4) Targeted Proteomic Sub-Core to provide high-
throughput, high- sensitivity analysis of cytokines, chemokines and other analytes with the use of Luminex Bio-
Plex technology and 5). Lipoprotein Sub-Core, which will measure lipid and lipoprotein levels in mouse plasma
and also provide standardized preparations of LDL and modified LDLs (and other needed lipoproteins) for
investigators’ use.
 The Core will be centered and organized in Dr. Witztum's laboratory at UCSD, who will be the overall
director and responsible to see that all of the Sub-Cores fulfill their missions. The Atherosclerosis Morphology
and Liver Morphology Sub-Cores will be housed at UCSD under the Direction of Dr. Witztum with assistance of
a UCSD expert in liver pathology (Dr. Tatiana Kisseleva), as will the services of the Lipoprotein Core. High-
throughput selected proteomic type analyses of cytokines, chemokines and related proteins will be performed at
the Salk Institute under the direction of Drs. Ron Evans and Michael Downes. Finally, the Lipid Maps Lipidomic
Core, under the direction of Drs. Oswald Quehenberger and Ed Dennis is also located at UCSD in the same
building as Dr. Witztum’s laboratories. Thus, all sample collection will be centralized in a central laboratory at
UCSD, where they will be logged into an Excel spreadsheet to provide a central organizational pathway for
distribution of samples to proper Sub-Cores and in turn, a central site for data collection of results.

## Key facts

- **NIH application ID:** 9936772
- **Project number:** 1P01HL147835-01A1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **Principal Investigator:** Joseph L. Witztum
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $415,844
- **Award type:** 1
- **Project period:** 2020-09-21 → 2025-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9936772

## Citation

> US National Institutes of Health, RePORTER application 9936772, PPG Phenotyping (1P01HL147835-01A1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9936772. Licensed CC0.

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