# Primary fibroblast resiliency as a predictor of health and lifespan in mice

> **NIH NIH R01** · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · 2020 · $309,396

## Abstract

In response to RFA-AG-17-040, “Short-term Measurements of Physical Resilience as a
Predictor of Healthspan in Mice”, we propose testing primary fibroblast resilience with a panel of
different cellular insults as a means to predict individual mouse longevity and healthspan. As outlined
by the funding announcement for this RFA, there is a need to develop these standardized tests for
use among the aging community to accelerate research towards revealing mechanisms that underly
the physiological decline of aging. We previously have shown that primary fibroblasts isolated from
the tail skin of mice likely retain characteristics of the in vivo environment of the mouse (or other
species) from which they were established. For example, we showed in a series of studies that skin-
derived primary fibroblasts isolated from long-lived mice with deficiencies in growth hormone/insulin-
like growth factor 1 levels are resilient to multiple cytotoxic and metabolic insults. These differences
persist even after numerous population doublings in culture using identical conditions as fibroblast
lines from control mice. In addition, we have shown in this fibroblast model that resiliency to one form
of insult predicts resiliency to multiple other forms of insult in an individual cell line. Our overall
hypothesis is that cellular resiliency of skin-derived primary fibroblasts represents the vitality of an
individual in vivo and predicts both healthspan and longevity of individual mice. We have designed
this study to test this hypothesis and meet the goals outlined by this RFA. In our first aim, we test
whether fibroblast resiliency is predictive of individual longevity and healthspan in a normally aging
group of genetically heterogeneous mice. Because of the unique fibroblast resiliency panel of tests
we have outlined, we can test physical resiliency of mice with little to no effect on the overall health
and longevity of the animals. That is, in an individual mouse we will measure fibroblast resilience
(including repeated assessments throughout middle age) and longevity and use these data to
develop a predictive model. In our second aim, we test the effect on fibroblast resiliency of
interventions in mice known to alter longevity and/or healthspan. This will test whether this model can
predict novel interventions that may alter these parameters within a population. Because we currently
lack standardized research tools to probe resiliencies at the cellular level, this marker of resilience
has the potential to be a highly important marker of healthspan and longevity in mouse studies.

## Key facts

- **NIH application ID:** 9937636
- **Project number:** 5R01AG057431-04
- **Recipient organization:** UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
- **Principal Investigator:** Adam Salmon
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $309,396
- **Award type:** 5
- **Project period:** 2017-09-15 → 2022-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9937636

## Citation

> US National Institutes of Health, RePORTER application 9937636, Primary fibroblast resiliency as a predictor of health and lifespan in mice (5R01AG057431-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9937636. Licensed CC0.

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