# Transgenic & Knockout Shared Resource

> **NIH NIH P30** · MAYO CLINIC ROCHESTER · 2020 · $115,987

## Abstract

TRANSGENIC AND KNOCKOUT SHARED RESOURCE PROJECT SUMMARY 
The main purpose of the Mayo Clinic Cancer Center Transgenic and Knockout (TKO) Shared Resource is to 
assist investigators at all 3 sites with the design and the generation of genetically modified mouse models. 
Utilization of the resource has been strong with 49 Cancer Center members from 8 different Programs using 
the Shared Resource during the current funding period (2013-2017). Our services include CRISPR/Cas9 
mediated editing of genes of interest in fertilized mouse eggs to generate knockout and knock-in mouse 
models to inactivate or tag it, or to introduce point mutations, reporter genes, loxP sites or large deletions. 
Although gene editing by CRISPR/Cas9 is incredibly useful, in some instances it is not as robust as 
conventional gene targeting in embryonic stem (ES) cells, prompting us to maintain our classical services 
mainly to insert large (>5 kb) DNA fragments and loxP sites. Additional services offered by the TKO Core 
include founder screening, founder breeding, sperm cryopreservation, recovery of cryopreserved mouse 
strains through in vitro fertilization, and standard histopathology. TKO Shared Resource staff also provide 
advice on DNA preparation, mouse genotyping, handling and husbandry. To stay at the cutting edge of 
genome editing, the TKO devotes time and effort to develop or implement innovative and experimental 
procedures. The above activities allow Cancer Center members to implement state-of-the-art mouse models to 
address key open questions in the field of cancer etiology and to design and test new therapeutic approaches 
that improve cancer treatment outcome. The TKO Shared Resource achieved a score of Outstanding at the 
previous site visit.

## Key facts

- **NIH application ID:** 9938451
- **Project number:** 5P30CA015083-46
- **Recipient organization:** MAYO CLINIC ROCHESTER
- **Principal Investigator:** MARK A. MC NIVEN
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $115,987
- **Award type:** 5
- **Project period:** 2020-03-01 → 2024-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9938451

## Citation

> US National Institutes of Health, RePORTER application 9938451, Transgenic & Knockout Shared Resource (5P30CA015083-46). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9938451. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*