# Advanced, multi-function, inverted confocal microscope for BNI Structural and Functional Imaging Core

> **NIH NIH S10** · WINIFRED MASTERSON BURKE MED RES INST · 2020 · $530,415

## Abstract

This application is a request for funds to purchase a Nikon A1R HD25 confocal microscope.
This microscope will be housed in the Structural and Functional Imaging Core at the Winifred
Masterson Burke Medical Research Institute (DBA Burke Neurological Institute; BNI). The Burke
Neurological Institute is a leading nonprofit scientific research institute with the mission to find
cures for chronic neurological disabilities. The mandate of the imaging core facility is to provide
access to a range of light microscopy systems and provide technical assistance with image
acquisition, processing, and analysis to all research groups within BNI. Confocal microscopy is
essential for the majority of the NIH-funded laboratories at BNI and the previous shared
confocal, a Zeiss LSM 510, has exceeded its serviceable lifetime. The Nikon A1R HD25 is both
a significant upgrade and a much-needed resource for the NIH-funded laboratories of BNI. The
A1R HD25 has many advanced features that would benefit our research progress; however,
there are three key features that make it most suitable for the diverse studies that are performed
in the Imaging Core: 1) the largest available working field of 25 mm, 2) the industry-leading
environmental chamber with multi-dish holder, temperature controller and built-in digital gas
mixer, and 3) Nikon's versatile Perfect Focus System (PFS), which corrects for focus drift
caused by temperature changes or mechanical disturbances. The institute is highly supportive
of this application and has pledged to invest in the maintenance and operation throughout its
useful lifetime, as it has done for 17 years on our previous multi-user confocal microscope. The
requested microscope will exert a sustained, powerful influence on the conduct of the current
NIH-funded research programs at BNI, and will be critical to the success of new studies as they
mature to NIH funding.

## Key facts

- **NIH application ID:** 9938816
- **Project number:** 1S10OD028547-01
- **Recipient organization:** WINIFRED MASTERSON BURKE MED RES INST
- **Principal Investigator:** Edmund R Hollis
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $530,415
- **Award type:** 1
- **Project period:** 2020-08-01 → 2021-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9938816

## Citation

> US National Institutes of Health, RePORTER application 9938816, Advanced, multi-function, inverted confocal microscope for BNI Structural and Functional Imaging Core (1S10OD028547-01). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9938816. Licensed CC0.

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