# BD FACSAria Fusion for the OHSU Knight Flow Cytometry Shared Resource

> **NIH NIH S10** · OREGON HEALTH & SCIENCE UNIVERSITY · 2020 · $599,918

## Abstract

PROJECT SUMMARY
 Funds are being requested to purchase a state-of-the-art BD Biosciences (BD) FACSAria Fusion
fluorescence activated cell sorter for the Oregon Health & Science (OHSU) Knight Flow Cytometry Shared
Resource. This shared resource is a university-wide core facility (Core) that is available to all OHSU
investigators. The proposed 5-laser 18-color instrument will replace a highly used, aging, maximally upgraded
BD FACSAria-I sorter that is no longer performing to the standards required by our investigators. For example,
sorted cell recovery has declined dramatically over the last year, despite frequent servicing by BD engineers and
daily maintenance and QA/QC assessments. Thus, the requested instrument will allow the Core to meet current
and future researcher needs. We are also requesting an upgrade to the instrument's biohazard containment
capabilities, to ensure safe operation by Core staff and investigators; and the addition of a UV laser, to enable
use for additional applications.
 The Core was established in 1996 and has been directed by Philip R Streeter, PhD, for the past 14 years.
Dr. Streeter works closely with the Core's 5 highly skilled flow cytometry operators to ensure delivery of the public
health related goals of the flow cytometric research of our many investigators. The Core serves a user base of
approximately 120 investigator laboratories, and provides advanced instrumentation, expertise, and
infrastructure to fully support research needs for analytical flow cytometry and cell sorting. Services offered
include: consultations, contributions to grant applications, education, training, data analyses, soluble analyte
quantification, analytical flow cytometry, and cell sorting. Hands-on training provides investigators with the
requisite tools and the background for comprehensive high-quality experimental design and data analyses.
Further, Core operators work closely with investigators to promote excellence in acquisition and analyses of flow
cytometric data.
 Cell sorting enables purification of live cell populations from complex cell mixtures. Cells can be sorted into
bulk populations or as single cells, and they can be deposited into wells, into tubes, or onto slides. Sorted cells
are used for in vitro cell culture; in vivo biological studies; or molecular, genetic, or proteomic analyses. Sorting
enhances the power of those technologies by yielding defined samples. Sorting enables investigators to
qualitatively and quantitatively assess cells tagged with fluorophore-labeled reagents or fluorescent proteins,
and provides investigators with the ability to isolate specified cell populations. The requested instrument is also
able to sort cell type specific extracellular vesicles, a technology being optimized for multiple applications at
OHSU. Acquisition of the requested instrument will support the Core's mission of delivering high quality flow
cytometric data to our many investigators, and it will facilitate delivery of the...

## Key facts

- **NIH application ID:** 9939101
- **Project number:** 1S10OD028512-01
- **Recipient organization:** OREGON HEALTH & SCIENCE UNIVERSITY
- **Principal Investigator:** PHILIP R STREETER
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $599,918
- **Award type:** 1
- **Project period:** 2020-07-15 → 2022-07-14

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9939101

## Citation

> US National Institutes of Health, RePORTER application 9939101, BD FACSAria Fusion for the OHSU Knight Flow Cytometry Shared Resource (1S10OD028512-01). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9939101. Licensed CC0.

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