# Nuclear, mitochondrial and L-A virus contributions to yeast quantitative traits

> **NIH NIH R01** · DUKE UNIVERSITY · 2020 · $413,400

## Abstract

Project Summary
Broad long-term objectives: In Specific Aim 1, we will determine the phenotypes (e.g. 7
mitochondrially targeted drugs; non-mitochondrially targeted drugs; growth at low and high
temperature) of 98 ± L-A strain pairs. We will identify strain pairs with strong L-A-dependent
phenotypes; that is, N1/N1 1 L-A0 ≠ N1/N1 1 L-A+. We will also identify candidate QTGs for
analysis in Aim 3. In Specific Aim 2, we will determine the phenotypes (e.g. 7 mitochondrially
targeted drugs; non-mitochondrially targeted drugs; growth at low and high temperature) of iso-
nuclear pairs of F1; for example, N1 1  N2 0  N1/N2 1 and N1 0  N2 2  N1/N2 2.
We will identify iso-nuclear F1 diploid strain pairs with strong mitochondrial genome-dependent
phenotypes; that is, N1/N2 1 ≠ N1/N2 2. We will cross strains with strong mitochondrial
genome-dependent phenotypes; for example, N1 1  N2 2  F1 N1/N2 ( recombinants). In
multiple F1 N1/N2 ( recombinants) diploids, we will identify phenotypically relevant
mitochondrial () QTG(s). In Specific Aim 3A, using our results from Aim 1 and 2, we will
perform RNA-Seq on ethanol and dextrose grown isogenic ± L-A parent strains and ± L-A- and
 genotype-controlled iso-nuclear F1. We will identify ± L-A and  genotype-dependent effects
on the transcriptomes and potential candidate QTGs, which will aid downstream analysis. In
Specific Aim 3B, we will (separately) sporulate multiple iso-nuclear F1 strain pairs with strong
L-A- and/or mitochondrial genome-dependent phenotypes (identified in Aim 1, 2; RNA-Seq in
Aim 3B) to generate ± L-A- and  genotype-controlled F12 populations. In multiple ± L-A F12
1 vs. ± L-A F12 2 population pairs, we will identify phenotypically relevant nuclear QTGs. We
will primarily focus on ± L-A and  genotype-specific nuclear QTGs to advance our
understanding of missing heritability, host-virus, and N- interactions.

## Key facts

- **NIH application ID:** 9939617
- **Project number:** 5R01GM118936-04
- **Recipient organization:** DUKE UNIVERSITY
- **Principal Investigator:** John H. McCusker
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $413,400
- **Award type:** 5
- **Project period:** 2017-08-16 → 2022-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9939617

## Citation

> US National Institutes of Health, RePORTER application 9939617, Nuclear, mitochondrial and L-A virus contributions to yeast quantitative traits (5R01GM118936-04). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9939617. Licensed CC0.

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