# Preclinical Assessment Core

> **NIH NIH P01** · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · 2020 · $391,250

## Abstract

Summary: The Preclinical Assessment Core will be comprised of three components: a hemodynamic subcore,
an imaging subcore, and a Biobank housing biological samples collected from an unique non-human primate
model of pulmonary arterial hypertension secondary to humanized simian immunodeficiency virus infection
(SIV-PAH) established and characterized in the previous funding cycle of the tPPG. All three components are
essential for the mechanistic and translational research goals of this tPPG application.
Specific Aims: The specific aims of the Preclinical Assessment Core are: 1.To provide expertise in novel
hemodynamic assessments of pulmonary hypertension and cardiac function in mouse and rat models of PAH
and pulmonary hypertension with heart failure and preserved ejection fraction (PH-HFpEF). 2. To provide
expertise in imaging-based studies for mechanistic and pathologic assessment of PAH and PH-HFpEF rodent
models. 3. To develop a Biobank from the primate model of SIV-PAH.
Core Services/Research Plan: The Hemodynamic subcore will provide assistance in the implementation of
rodent animal models of PAH and PH-HFpEF, biventricular hemodynamic assessment by Doppler flow and
pressure-volume measurements, and echocardiographic measurements for non-invasive evaluation of cardiac
structure and function in rodents with PAH and PH-HFpEF. The Imaging component will provide expertise in
the preparation, storage and immuno-labeling of tissues in living and fixed samples from animal models. These
will include identification of key signaling pathways and characterization of disease pathology and progression
by quantitative measures of pulmonary vascular and heart remodeling using image analysis approaches. The
Core will provide access to a Biobank of lung, heart tissue, pulmonary artery smooth muscle cells (PASMC),
plasma, bronchoalveolar lavage, and fecal samples from different time points of the SIV-PAH primate model
that will allow functional correlations and mechanistic studies from early to advanced stages of the disease.
PASMC will be used to develop an in vitro model to evaluate the effect of metabolic disorders and therapeutic
candidates in PASMC proliferation.
Significance and synergy: The Preclinical core will support studies in animal models of PAH and PH-HFpEF
that closely recapitulate the pathogenesis and clinical outcome of human disease, and more importantly, have
proven value in evaluating new therapies. All Projects will use resources of the Preclinical Assessment Core to:
1) test mechanistic hypotheses of the pathogenesis of PAH and PH-HFpEF, 2) test the efficacy of new
therapeutics in established rodent models of PAH and PH-HFpEF, and 3) evaluate molecular mechanisms in
biological samples from the SIV-PAH model. In addition, the Preclinical Core will synergize with the repository
of human samples from the Clinical Core to serve as an integrated resource for validation of pulmonary
hypertension target molecules as well as mechanistic pathway...

## Key facts

- **NIH application ID:** 9939657
- **Project number:** 5P01HL103455-10
- **Recipient organization:** UNIVERSITY OF PITTSBURGH AT PITTSBURGH
- **Principal Investigator:** Ana Lucia Mora
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $391,250
- **Award type:** 5
- **Project period:** 2011-06-01 → 2023-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9939657

## Citation

> US National Institutes of Health, RePORTER application 9939657, Preclinical Assessment Core (5P01HL103455-10). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9939657. Licensed CC0.

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