# Q Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer (QE HF-X)

> **NIH NIH S10** · CASE WESTERN RESERVE UNIVERSITY · 2020 · $600,000

## Abstract

Project Summary / Abstract
In this grant proposal the Center for Proteomics and Bioinformatics (CPB) will acquire, install, and operate an
Q Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer (QE HF-X) and ultra high pressure liquid
chromatography system optimal for a wide range of structural proteomics, proteome expression, and
phosphoproteomics studies. The CPB has assembled a robust research base for the use of this instrument
that includes 14 funded projects with 13 PIs/Co-Is who have over $6.8M of direct costs funding per year in their
peer-reviewed grants. For these projects to fulfill their full potential and remain competitive over time cutting
edge instrumentation and data, such as that provided by the proposed instrument are essential. These
research teams have extensive prior experience with the CPB and the user groups have leveraged proteomic
data and research with exemplary publications and funding in the proteomics field. The Center also operates
proteomics core for the Case Center for AIDS Research and the Case Cancer Center both of which are peer-
reviewed Cores of major funded NIH funded Centers. The PI and CPB faculty and staff have long-standing
experience with installing and operating advanced MS instrumentation assuring that the proposed instrument
will have excellent stewardship and that allocations of instrument time will be fair and transparent resulting in a
high level of productivity for the research base in Cleveland and across the US including outreach to under
represented groups in science. The CPB's overall MS-based analytical portfolio is very diverse, providing
cutting edge technologies to users across the university and beyond spanning small molecule, structural, and
quantitative proteomics. For example, last year the CPB supported 48 different user groups many with multiple
projects (120 separate projects). These users spanned 12 University and Hospital departments (CC & UH)
along with outside users, such as industry (4%) or other local or national universities (15%). As user needs
and the proteomics field has evolved there is increasing demand for higher resolution, higher
sensitivity, and higher mass accuracy mass spectrometry (MS) for structural proteomics and other
quantitative proteomics studies (particularly phosphoproteomics) that require identification and
quantification of peptides modified at specific side chains. The Core currently utilizes 2 Thermo Elite
Orbitrap mass spectrometers coupled to Nano-Acquity ultra high-pressure liquid chromatography systems
(UPLC) for these quantitative and structural proteomics studies. Unfortunately, the current high resolution
instruments in the Core lack fast duty cycles and ultra-efficient ion optics now available for detection of low
abundance species and chemical modifications of side chains observed for footprinting. The instruments are
also 8 years old and are no longer manufactured by the vendor. Thus, the existing pipelines would have
considerably impr...

## Key facts

- **NIH application ID:** 9939958
- **Project number:** 1S10OD028614-01
- **Recipient organization:** CASE WESTERN RESERVE UNIVERSITY
- **Principal Investigator:** MARK R CHANCE
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $600,000
- **Award type:** 1
- **Project period:** 2020-09-01 → 2021-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9939958

## Citation

> US National Institutes of Health, RePORTER application 9939958, Q Exactive HF-X hybrid quadrupole-Orbitrap mass spectrometer (QE HF-X) (1S10OD028614-01). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/9939958. Licensed CC0.

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