# Amnis ImageStreamX MarkII Imaging Flow Cytometer

> **NIH NIH S10** · UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH · 2020 · $517,163

## Abstract

Project Summary/Abstract
This proposal is a request for funding for an Amnis ImageStreamX MarkII Imaging Flow
Cytometer by Luminex Corporation. The instrument will be permanently placed within the
University of Utah Flow Cytometry Core Facility. All operational oversight will be carried out by
the qualified staff of the Flow Facility and its established management structures.
As an analytical instrumentation core facility, we strive to provide intellectual expertise in
cytometry as well as the physical tools needed by a broad range of biological disciplines to
answer a widening array of inquiries. More specifically, for a cytometrist, this translates into
measuring both intrinsic and extrinsic properties of cells within a heterogeneous population.
Historically, microscopy has been the source of definitive fluorescent localization and
morphological information, while flow cytometry has carved a niche of high throughput, high
parameter, suspension cell analysis. Unfortunately, neither of these indispensible technologies
has been capable of providing the full breath of data needed for a sizable set of specialized
applications. These applications predominantly consist of suspension or non-adherent cellular
analysis, where the target cell of interest is limiting and some level of morphometrical or
localization evaluation is essential. The Amnis ImageStreamX represents a true crossover
platform that can provide high throughput quantitative data assessing both of these attributes,
and many more. Described within this application is a wide range of NIH funded research
projects that will uniquely benefit from the diverse analytical capabilities of the ImageStreamX.
This platform will greatly advance standardization, reproducibility, and throughput, therefore
increasing the quality and speed of scientific progress. Additionally, this instrument has the
unmatched ability to combine morphological and protein localization differences with flow
cytometry fluorescence gating to isolate populations that researchers have never been able to
isolate before. Finally, placement of this instrument into a highly respected shared resource lab
with dedicated trained staff will ensure its long term operational availability and financial stability.

## Key facts

- **NIH application ID:** 9940051
- **Project number:** 1S10OD026959-01A1
- **Recipient organization:** UTAH STATE HIGHER EDUCATION SYSTEM--UNIVERSITY OF UTAH
- **Principal Investigator:** James E Marvin
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $517,163
- **Award type:** 1
- **Project period:** 2020-09-01 → 2021-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9940051

## Citation

> US National Institutes of Health, RePORTER application 9940051, Amnis ImageStreamX MarkII Imaging Flow Cytometer (1S10OD026959-01A1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9940051. Licensed CC0.

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