# Super-resolution Imaging via Multiple Modalities with the Zeiss Elyra 7

> **NIH NIH S10** · DUKE UNIVERSITY · 2020 · $694,950

## Abstract

ABSTRACT
The microscope we propose in this application is the new Zeiss Elyra 7 system, which combines multiple
modes of super-resolution imaging. The new Lattice SIM technology of the Elyra 7 enables structured
illumination super-resolution imaging of common fluorescent proteins and labels, even in tissue samples, due
to the increased signal to noise imaging with the lattice pattern. Increased light efficiency allows 2x diffraction
limited imaging at high speed with less phototoxicity and bleaching – up to 255 fps.
One can examine the
fastest processes in living samples – in large fields of view, in 3D, over long time periods, and with multiple
colors.
The Elyra 7 also has single molecule localization microscopy (SMLM) capabilities for techniques such
as photoactivation localization microscopy (PALM), direct stochastic optical reconstruction microscopy
(dSTORM) and points accumulation for imaging in nanoscale topography (PAINT) with high power laser lines
and dual camera functionality.
The new Apotome mode gives superfast optical sectioning of 3D samples with
minimal photobleaching. We note PALM/STORM and lattice-structured illumination of the Elyra 7 includes
Zeiss software solutions for the analysis of images acquired in each of these modes and thus adds
capabilities in super-resolution imaging that currently do not exist on Duke campus. The flexibility and new
technologies of the ELYRA 7 will significantly contribute to new research capabilities related to NIH funded
developmental biology, neuroscience, and cell biology questions relevant to human disorders as outlined in
the research statement. The instrument would be housed in a large shared resource serving the entire
research base at Duke University and Duke School of Medicine where the impact and use would be
widespread. The LCMF Director has experience with these forms of imaging and has general high-levels of
imaging expertise and so is well placed to immediately and effectively help implement these methods for an
important array of basic research. Additionally, as part of our broad long-term objectives with institutional
support, the ELYRA 7 works seamlessly with ZEISS SEMs in a correlative light-EM imaging workflow via
integrated software solutions that enable the precise landmarking of light imaging regions of interest to be
automatically recalled from the same sample for electron microscopy. This added benefit will be paradigm
shifting for many research programs in our user group.

## Key facts

- **NIH application ID:** 9940085
- **Project number:** 1S10OD028703-01
- **Recipient organization:** DUKE UNIVERSITY
- **Principal Investigator:** SCOTT H SODERLING
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $694,950
- **Award type:** 1
- **Project period:** 2020-07-10 → 2021-07-09

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9940085

## Citation

> US National Institutes of Health, RePORTER application 9940085, Super-resolution Imaging via Multiple Modalities with the Zeiss Elyra 7 (1S10OD028703-01). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9940085. Licensed CC0.

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