# Microparticle Sorting for the Study of New Immunotherapies

> **NIH NIH S10** · CASE WESTERN RESERVE UNIVERSITY · 2020 · $599,902

## Abstract

ABSTRACT
The Case Comprehensive Cancer Center Cytometry & Imaging Microscopy Core provides access to
cytometry, cell sorting, and microscopic instruments and services to the campus of Case Western Reserve
University (CWRU) and the wider Cleveland area. This core has been in continuous existence since 1985 and
funded by the NCI through a P30 grant since 1987. In recent years, the core has formed cooperative
agreements with the Case Center for Aids Research (CFAR) Immune Function Core, and the Digital Imaging
Microscopy Core of the Cleveland Clinic Lerner Research Institute to leverage complimentary expertise and
reduce instrument duplication.
Within the past four years, over 500 users from 195 laboratories have used the core. Of these, 42 NIH funded
investigators need continuous cell sorting support. The core has two cell sorters; the main instrument is aged
BD Aria with limited laser/parameter capability, and the second sorter is a Sony Reflection which is obsolete,
operationally unstable (and therefore not safe for BSL II work), and has limited multiparameter work. Because
of the growing need for sorting per se, sorting of viable human cells, infected cells, or other BSL II samples,
and increased need by investigators with fragile and/or time-sensitive samples, we need to replace the
Reflection with a modern cell sorter that matches the capability of our analytical instruments. We propose to
acquire a BD Influx equipped with a laser/pmt/filter set up that mimics our LSR II and Fortessa instruments and
additionally has microparticle sorting capability to sort virus, exosomes, and high throughput sorting of
Circulating Tumor Cells. This will provide stable, safe, and flexible sorting capability and sufficient capacity for
this large, busy core. This BD Influx will shore up failing systems as well as greatly expand the capacity and
cutting edge research being performed in our area.

## Key facts

- **NIH application ID:** 9940282
- **Project number:** 1S10OD026841-01A1
- **Recipient organization:** CASE WESTERN RESERVE UNIVERSITY
- **Principal Investigator:** Brian T. Grimberg
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $599,902
- **Award type:** 1
- **Project period:** 2020-09-05 → 2021-09-24

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9940282

## Citation

> US National Institutes of Health, RePORTER application 9940282, Microparticle Sorting for the Study of New Immunotherapies (1S10OD026841-01A1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9940282. Licensed CC0.

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