# Request for Abberior easy 3D-STED Super-Resolution Microscope

> **NIH NIH S10** · EMORY UNIVERSITY · 2020 · $1,056,967

## Abstract

This application requests funds to purchase an Abberior FACILITY LINE easy3D-STED fluorescence microscope
to be included in the existing Integrated Cellular Imaging Core (ICIC). The primary goal of the ICIC is to provide
investigators at Emory and surrounding Georgia institutions access to cutting edge light-based microscope
technology, services, and expertise. This open shared resource has assisted 175 NIH grants held by 192
investigators in the last 2 FYs. Within the facility, the Abberior STED microscope will be used to study diverse
aspects of molecular level organization pertaining to health and disease that include: axonal regeneration
(Alvarez), muscle sarcomere function (Benian), mitochondrial regulation (Kahn), epithelial barrier function
(Koval), cell junctions (Kowalczyk), viral genomic fitness (Lowen), viral entry and uncoating (Melikian), antiviral
development (Sarafianos), MSC-induced immune regulation (Horwitz), retinal pigment epithelium (Nickerson)
and mitochondrial membrane potential (Kenney).
The Abberior easy3D-STED combines a unique balance of real-time super-resolution, ease of use, flexibility,
and speed, thus providing researchers with expedited answers to their disease related molecular level questions.
Adjustable real-time 3D super-resolution incorporated into a familiar laser scanning confocal microscope makes
this system one of the most approachable super-resolution microscopes. Flexible ultra-sensitive photon-
counting four-spectral-channel detection provides a sustained application across a busy core facility within an
evolving experimental landscape. This resolution, ease of use, and sensitivity coupled with adaptive illumination
controlled and drift-compensated STED makes 4-color real-time live-cell 3D imaging at the nanoscale now
possible, providing huge impact for the research community in an almost unlimited number of investigations.
This microscope will be managed as part of the facilities under the same stringent policies to ensure optimal
usage and minimize downtime. Our facility has a strong financial commitment from the School of Medicine and
Winship Cancer Institute, including deficit coverage, seed funding, and pilot grants. We feel the Abberior STED
is an essential tool for addressing both current and future research needs of a largely NIH funded research
community at Emory and surrounding institutions.

## Key facts

- **NIH application ID:** 9940419
- **Project number:** 1S10OD028673-01
- **Recipient organization:** EMORY UNIVERSITY
- **Principal Investigator:** Adam I. Marcus
- **Activity code:** S10 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $1,056,967
- **Award type:** 1
- **Project period:** 2020-06-15 → 2021-06-14

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9940419

## Citation

> US National Institutes of Health, RePORTER application 9940419, Request for Abberior easy 3D-STED Super-Resolution Microscope (1S10OD028673-01). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9940419. Licensed CC0.

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