# Molecular Biology and Lipidomics Core - Core B

> **NIH NIH P01** · UT SOUTHWESTERN MEDICAL CENTER · 2020 · $540,167

## Abstract

Core B  Molecular Biology and Lipidomics provides multiple essential services to every investigator
working on this Program Project Grant. These services include routine experimental procedures as well as
unique assays that provide unparalleled and comprehensive molecular insight into lipid metabolism. The core
is directed by Dr. David W. Russell, who has served as its Principal Investigator for the past 30 years.
 Routine services include protein gel electrophoresis and immunoblotting; DNA sequencing of wild-type
and mutant cDNAs and plasmid constructs; RNAseq assays to assess the transcriptomes of cells and tissues;
quantitative PCR assays to measure mRNA levels expressed from hundreds of mouse, human, and hamster
genes; the design and ordering of custom oligonucleotide probes and primers; the preparation of genomic and
plasmid DNAs for experimentation and storage; and the assessment of physiological parameters (oxygen
consumption, carbon dioxide production, hemodynamic parameters, weight gain and loss, activity, etc.) in living
animals.
 Unique assays developed through the auspices of this Program Project include mass spectrometry-
based quantification of individual lipid species including sterols, oxysterols, ceramides, steroids, bile acids, and
fatty acids; lipidomic profiling that yields qualitative to semi-quantitative assessments of thousands of lipid
molecular species; and flux measurements that quantify the flow of metabolites through a designated
biosynthetic or catabolic pathway in cultured cells and living animals.
 The extensive and expensive equipment needed to provide these services, including multiple mass
spectrometers, liquid-handling robots, metabolic cages, freezers, real-time PCR machines, and routine and
next-generation DNA sequencers, is provided solely by the institution and represents a substantial cost-sharing
investment.
 The services provided by this Core play a major role in every specific aim in this Program Project and
in every publication that arises from the completed research.
 C/PPG 2015 – Core B – Project Summary

## Key facts

- **NIH application ID:** 9940754
- **Project number:** 5P01HL020948-44
- **Recipient organization:** UT SOUTHWESTERN MEDICAL CENTER
- **Principal Investigator:** DAVID W. RUSSELL
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $540,167
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9940754

## Citation

> US National Institutes of Health, RePORTER application 9940754, Molecular Biology and Lipidomics Core - Core B (5P01HL020948-44). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9940754. Licensed CC0.

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