# Core C:  Proteomics and Analytical Biochemistry Core

> **NIH NIH P01** · UNIVERSITY OF ILLINOIS AT CHICAGO · 2020 · $330,514

## Abstract

Core C: Summary/Abstract
The proteomics and analytical biochemistry core C provides essential technical support for all three of the
projects in our program. The main functions of Core C are to provide: (i) support for determining post-
translational modifications (PTM) in cardiac tissue (human from Core B, rodent and rabbit) with an emphasis
on sarcomeric proteins utilizing 2D-DIGE, 2D-PAGE and Pro-Q staining; (ii) separation of cardiac proteins or
peptides in solution by OFFGEL fractionation; (iii) mass spectrometric (MS) analysis support to identify proteins
and their (PTMs) along with multiple reaction monitoring (MRM) for targeting specific PTMs; (iv) support for
identifying reactive oxygen species (ROS) induced protein modifications by Oxyblot, 2D-diagonal gels,
glutathionylation Western blot assays and biotin switch assays; (v) support for determining global changes in
both protein abundance and PTMs by iTRAQ/TMT labeling of either the whole proteome or sub-proteomic
fractions. Core C also prepares sub-proteomic fractionation of the sarcomeric proteins or whole heart
homogenates for proteomic characterization. To ensure productivity and progress, Core C support provides
state-of-the-art facilities and equipment such as the Orbitrap Velos Pro and the AB Sciex 6500 Q-TRAP for
advanced proteomic analysis required by all projects in the program. Core C support is critical to approach for
aims in each of the projects and thus is indispensable for the overall success of the program.

## Key facts

- **NIH application ID:** 9941108
- **Project number:** 5P01HL062426-20
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT CHICAGO
- **Principal Investigator:** Chad Michael Warren
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $330,514
- **Award type:** 5
- **Project period:** — → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9941108

## Citation

> US National Institutes of Health, RePORTER application 9941108, Core C:  Proteomics and Analytical Biochemistry Core (5P01HL062426-20). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9941108. Licensed CC0.

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