# Molecular Signatures

> **NIH NIH U19** · SALK INSTITUTE FOR BIOLOGICAL STUDIES · 2020 · $2,466,377

## Abstract

Abstract
Understanding the exact cell-type composition in the different regions of the mouse brain is a fundamental step
when trying to integrate physiological, behavioral, neurochemical and molecular data. At present, although
major categories of cell-types present in the mouse brain have been defined through a handful of specific
markers, the different subtypes within these categories, as well as their location and connectivity are far from
understood. Epigenomic signatures such as DNA methylation (mC) and open chromatin are stable
modifications that persist in post-mitotic cells throughout their lifetime, defining their cellular identity. Open
chromatin as well as methylation patterns are cell type specific, differentiating the major types of cells i.e.,
neurons and glia, in the rodent and human cortex, as well as differentiating neuronal types in mouse brain.
Research Segment 1 of this center proposes to produce a catalog of methylation and open chromatin patterns
at the single-cell level throughout the entire mouse brain. Analysis of the combined data will permit the
discovery of cell-type specific regulatory regions that will allow the production of transgenic mouse lines and
viral tools that will become available to the community.

## Key facts

- **NIH application ID:** 9941137
- **Project number:** 5U19MH114831-04
- **Recipient organization:** SALK INSTITUTE FOR BIOLOGICAL STUDIES
- **Principal Investigator:** Joseph R Ecker
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $2,466,377
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9941137

## Citation

> US National Institutes of Health, RePORTER application 9941137, Molecular Signatures (5U19MH114831-04). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/9941137. Licensed CC0.

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