# Mass Spectrometry

> **NIH NIH P30** · JOHNS HOPKINS UNIVERSITY · 2020 · $72,050

## Abstract

ABSTRACT
The Sidney Kimmel Comprehensive Cancer Center (SKCCC) Mass Spectrometry Core is a part of the larger
institutional Johns Hopkins University School of Medicine (JHUSOM) Mass Spectrometry and Proteomics
Facility. It provides SKCCC investigators access at a reduced cost to advanced proteomic services, a
designated Core Mass Spectrometrist and a Proteomics Specialist, a specific biostatistician/bioinformatician
for post-identification/quantification data analysis. The Mass Spectrometry Core couples multidimensional
separations by column chromatography or gel electrophoresis to mass spectrometry analysis. It is used to
identify, quantify and characterize proteins and their post-translational modifications expressed in well-
characterized protein fractions from cancerous cells, tissues or body fluids. Techniques such as isobaric
mass tag labeling (TMT, tandem mass tags, or iTRAQ, isobaric tag for relative and absolute quantitation),
stable isotope labeling of amino acids in cell culture (SILAC) and nonlabeling methods (MuDPIT,
multidimensional protein identification technology) are available for quantifying relative differences in protein
expression and post-translational modifications, such as acetylation, AMPylation, citrullination, glycosylation,
phosphorylation, nitrosation, proline hydroxylation, ubiquitination and novel cleavage sites. The Core offers
recently established targeted proteomic methods, such as absolute quantification (AQUA) and protein
standard absolute quantification (PSAQ) methods, to determine the amount of specific proteins. In addition,
the Core offers high-resolution mass and fragmentation analysis of intact proteins—a top down approach to
characterize proteins and their modifications. Core services include pre- and post-analysis consultation on
sample preparation, data analysis and interpretation; proteolytic digestion, peptide extraction, labeling and
fractionation; nanoliquid chromatography separation coupled with tandem mass spectrometry analysis;
database searching; compiled results reports; and educational programs through core presentations and
technical workshops. A biostatistician/bioinformatician is available when more in-depth statistical and
ontological analyses of the proteomic results are needed. When SKCCC investigators wish to perform their
own analysis, the Core provides access to proprietary software and equipment. Core workshops on
operating the Core MALDI mass spectrometer are offered several times a year. During this funding period,
57 SKCCC investigators have used the Mass Spectrometry Core, and more than 50 manuscripts are
attributed to this Core.
Jointly SKCCC and Department of Biological Chemistry Managed Core
Reporting Period: Jan. 1, 2015, to Dec. 31, 2015

## Key facts

- **NIH application ID:** 9944491
- **Project number:** 5P30CA006973-57
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **Principal Investigator:** Robert N Cole
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $72,050
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9944491

## Citation

> US National Institutes of Health, RePORTER application 9944491, Mass Spectrometry (5P30CA006973-57). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9944491. Licensed CC0.

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