# Transcriptional control of motor neuron maturation

> **NIH NIH R01** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2020 · $376,783

## Abstract

Our ability to produce neurons from pluripotent stem cells holds great promise for studying the assembly,
function, and dysfunction of the nervous system in experimentally accessible settings. While there has been a
dramatic advance in the development of new protocols for differentiation of stem cells into increasingly diverse
types of neurons, stem cell-derived neurons fail to acquire a fully mature neuronal identity. In order to study the
function of mature nerve cells and to model adult onset neurodegenerative disorders in a more relevant context,
it is paramount to develop methodologies that yield nerve cells more closely resembling those found in the adult
CNS. Towards this goal, we will perform a longitudinal study of gene expression and chromatin changes in
primary motor neurons in the mouse spinal cord. We will identify enhancers that control mature gene expression
programs and develop methods for reprograming immature stem cell-derived motor neurons to a mature state.
This work will impact three important areas: 1) it will provide the first detailed analysis of gene expression
changes associated with motor neuron maturation in vivo, 2) it will define new transcriptional regulators
controlling neuronal maturation, and 3) it will yield a new effective method for reprogramming immature stem
cell-derived motor neurons to a more mature state that will serve as a better model for adult-onset
neurodegenerative diseases, such as amyotrophic lateral sclerosis.

## Key facts

- **NIH application ID:** 9945271
- **Project number:** 1R01NS116141-01
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Hynek Wichterle
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $376,783
- **Award type:** 1
- **Project period:** 2020-03-01 → 2025-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9945271

## Citation

> US National Institutes of Health, RePORTER application 9945271, Transcriptional control of motor neuron maturation (1R01NS116141-01). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/9945271. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*