# Multiplexed Pathogen Detection from Whole Blood for Rapid Detection of Sepsis

> **NIH NIH R21** · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 2020 · $194,355

## Abstract

Multiplexed Pathogen Detection from Whole Blood for Rapid Detection of Sepsis
Abstract
Sepsis, an adverse auto-immune response to an infection often causing life-threatening
complications, results in the highest mortality and treatment cost of any illness in US hospitals.
In this proposal we propose a novel microchip to bridge the existing gaps in the pathogen
detection (poor sensitivity and long processing time).
Our strategy in the proposed work is performing picolitre volume RT-LAMP reactions on dried
blood using our in-house developed bi-phasic reaction to achieve a highly sensitive pathogen
count (1-3 CFU/mL).
The realization of this microwell array platform potentially overcomes the limitations in
sensitivity and time of response of the current blood cultures. We expect that this device will
greatly contribute to the faster rapid diagnosis of sepsis. As a final goal, we anticipate that this
platform will replace the use of blood culture for pathogen detection.

## Key facts

- **NIH application ID:** 9947885
- **Project number:** 5R21AI146865-02
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
- **Principal Investigator:** Rashid Bashir
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $194,355
- **Award type:** 5
- **Project period:** 2019-06-06 → 2021-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9947885

## Citation

> US National Institutes of Health, RePORTER application 9947885, Multiplexed Pathogen Detection from Whole Blood for Rapid Detection of Sepsis (5R21AI146865-02). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/9947885. Licensed CC0.

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