# Regulation of type II cells in the repair of alveolar epithelial injury

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT CHICAGO · 2020 · $399,750

## Abstract

Title: Regulation of type II cells in the repair of alveolar epithelial injury
ABSTRACT
Each year in the United States, there are ~ 200,000 cases of acute lung injury (ALI) and acute respiratory
distress syndrome (ARDS) . Repair of the injured alveolar epithelial barrier is essential for the resolution of
ALI/ARDS. However, there is currently a lack of targeted therapies aimed at promoting the repair of the
epithelium to restore barrier function. The goal of this project is to understand the signaling events contributing
to the endogenous alveolar repair process and identify potential drug targets to accelerate repair. Lung alveoli
are lined with two types of epithelial cells: Alveolar Type I cells (AT1) and Type II cells (AT2). AT1 cover ~95%
of the surface area because of their thin and squamous shape and are responsible for blood-gas exchange.
AT2 have multiple functions including the secretion of surfactant and are also able to act as progenitor cells,
proliferating and converting to AT1 after lung injury to restore the alveolar epithelial barrier. However, the
signaling events responsible for regulating AT2-mediated repair remain poorly understood. Our preliminary
data demonstrated the requirement of a non-canonical Notch ligand Dlk1 (For delta-like 1 homolog) in the AT2
to AT1 differentiation. We found that a dynamic change in Dlk1 expression is correlated with the AT2 to AT1
transition during repair. Using a mouse model in which Dlk1 was specifically disrupted in AT2, we found that
the mutant cells were unable to differentiate into AT1 and at mean time had abnormally elevated Notch
signaling. Based on these data, we hypothesize that dynamically regulated Notch signaling is essential
for the proper progenitor function of AT2 and that Dlk1 plays an essential role in mediating AT2 to AT1
transition during alveolar repair by inhibiting Notch signaling in a population of intermediate cells. We
intend to pursue the following specific aims: Aim 1: To test the hypothesis that Dlk1 regulates AT2 to AT1
cell transition and thereby mediates alveolar repair. We will determine the function of Dlk1 in restoring
epithelial barrier integrity and gas-exchange function post-injury. We will define the detailed steps and
molecular mechanisms in the Dlk1 regulated AT2 to AT1 transition. Aim 2: To test the hypothesis that Dlk1-
dependent dynamic regulation of the Notch signaling pathway mediates AT2-dependent alveolar
repair. We will determine the role of Notch signaling activity in AT2 at different phases of alveolar repair. Aim
3: To test the hypothesis that Dlk1 dependent Notch signaling promotes the differentiation of human
AT2 into AT1. At the conclusion of these studies, we will have filled an important gap in knowledge about the
molecular mechanisms underlying alveolar repair. We believe that these studies will lead to the discovery of
therapeutic targets to accelerate lung repair and prevent chronic pathological conditions resulting from
improper recove...

## Key facts

- **NIH application ID:** 9948720
- **Project number:** 5R01HL105947-08
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT CHICAGO
- **Principal Investigator:** YURU LIU
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $399,750
- **Award type:** 5
- **Project period:** 2012-05-01 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9948720

## Citation

> US National Institutes of Health, RePORTER application 9948720, Regulation of type II cells in the repair of alveolar epithelial injury (5R01HL105947-08). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9948720. Licensed CC0.

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