# Shared Resource Core: Single Cell Analysis

> **NIH NIH U54** · UNIVERSITY OF CALIFORNIA-IRVINE · 2020 · $222,791

## Abstract

The Cancer Systems Biology Center will develop a new core, the Single-Cell Analysis
Core. This core will provide technical, bioinformatic, and training support for the
Center. Cancers represent heterogeneous collections of cells interacting within the
cancer, but also affecting the host through systemic signaling and metastatic expansion.
High-throughput sequencing of single cells provides direct insight into the complexity of
the cancer cell population. The high-throughput, but noisy data generated from single-
cell experiments presents new challenges to investigators. Similar to the advent of other
breakthrough technologies, diverse approaches and innovative analysis must
accompany technological development. The goal of Core technical support is to make
cost-effective high-throughput analysis of single cells optimal for and accessible to each
of the three Projects. Technical support will consist of providing staff and instrument
infrastructure, as well as advising on design of experimental strategies, facilitating
sharing of protocols for process development; providing single-cell services including
microscopy, protein localization, cell sorting, library production, and sequencing; and
working with investigators to innovate in these technologies. The goal of bioinformatic
support by the Core is to develop analysis pipelines optimized as appropriate for the
three main Projects; mediate active sharing of data from the projects through uniform
reporting standards and a shared server; and finally fostering awareness of
technological advances within the Center and throughout the broader systems and
cancer communities. Training support for the Center is one of the most critical Core
functions, necessitated by the challenging pace of technological change. The Core will
advise and survey Center members as to target areas for workshops open to students,
fellows, staff, and faculty. Workshops will range in focus from wet bench workshops for
cell isolation and sorting; custom library production, C1 and Chromium platform walk-up
use, and microscopic screening of cell samples for sequencing to bioinformatic
workshops on single-cell commercial software (C1 Fluidigm software; Cell Ranger; and
Ingenuity Pathway Analysis etc.) and open source analysis software and statistical
packages for single cells. Finally, the Single-Cell Analysis Core will establish a biweekly
Cancer Single-Cell Research Meeting in which both current literature and research in
progress will be discussed. The new Single-Cell Analysis Core will promote and
integrate the progress of the three research Projects.

## Key facts

- **NIH application ID:** 9949387
- **Project number:** 5U54CA217378-03
- **Recipient organization:** UNIVERSITY OF CALIFORNIA-IRVINE
- **Principal Investigator:** SUZANNE SANDMEYER
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $222,791
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9949387

## Citation

> US National Institutes of Health, RePORTER application 9949387, Shared Resource Core: Single Cell Analysis (5U54CA217378-03). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9949387. Licensed CC0.

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