# Analytical Core

> **NIH NIH P01** · UNIVERSITY OF VIRGINIA · 2020 · $374,103

## Abstract

Analytical Core B Abstract
The Analytical Core (Core B) will be organized to provide high quality, reproducible, low cost, automated high-
throughput robotic analytical services to the three Projects. The Core will be managed and staffed with experts
who have demonstrated an ability to develop, engineer, and implement the assays necessary to support the
proposed activities of the Analytical Core. The Analytical Core will be organized around 5 major
services/specific aims.
Specific Aim 1: To provide high quality, reproducible automated assays to support Projects 1-3.
Specific Aim 2: To provide genotyping services for Projects 1 and 2. We will test for the presence of single
nucleotide polymorphisms (SNPs) in the subjects recruited under Project 2.
Specific Aim 3: To provide human renal proximal tubule (RPT) cells as well as renal distal tubule (RDT)
and renal collecting duct (RCD) cells for Projects 1 and 3. We will also isolate, immortalize and culture RPT,
RTD and RCD cells from fresh human urine specimens from Project 2. Mouse urine cells will be obtained from
Project 3.
Specific Aim 4: To provide cellular imaging services to support Projects 1 and 3. In addition, in support of
Project 2, we will study cells from the urine of subjects treated as outlined in the protocols of Project 2. We will
also study cells isolated from mouse urine obtained from Project 3.
Specific Aim 5: To provide flow cytometry services to support Projects 1-3.
1. Robotic Analytical Platforms
High Throughput Assays
High Content Assays
5. Flow Cytometry
Accuri Urine Cell Assays
Analytical
 Core B
2. Molecular Biology:
SNPs for salt sensitivity and
hypertension
DNA extraction and Biobanking
4. Confocal Microscopy:
FRET pH Assays
Cytoplasmic cAMP, cGMP sensors
Intracellular Na+ fluorescence
3. Cell Culture:
RPT, RDT, RCD cell lines from
human kidney tissue and urine
Microcarrier-3D culture

## Key facts

- **NIH application ID:** 9949769
- **Project number:** 5P01HL074940-15
- **Recipient organization:** UNIVERSITY OF VIRGINIA
- **Principal Investigator:** JOHN Joseph GILDEA
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $374,103
- **Award type:** 5
- **Project period:** — → 2022-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9949769

## Citation

> US National Institutes of Health, RePORTER application 9949769, Analytical Core (5P01HL074940-15). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/9949769. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*