# Outer and Inner Hair Cell Development

> **NIH NIH R01** · NORTHWESTERN UNIVERSITY · 2020 · $634,782

## Abstract

Project Summary
The cochlea contains two types of mechanosensory receptor cells, inner (IHC) and outer (OHC) hair cells,
which play very different, but critical, roles in hearing. Cochlear hair cells arise during embryogenesis, but how
they specifically differentiate into IHCs or OHCs remains unknown. We found a presumed transcription factor
(INSM1) expressed in incipient OHCs, but not in IHCs, during mouse embryogenesis. Insm1 is the first gene
found to be uniquely expressed in nascent, early differentiating OHCs (but not IHCs). We find that ablating
Insm1 affects OHCs and will elucidate its role in OHC differentiation. We hypothesize that INSM1 acts by
regulating (either activating or repressing) other genes early in OHC differentiation and will identify these target
genes. Finally, we will take advantage of a unique reporter line (Insm1GFP::Cre) that selectively labels OHCs from
their onset to cell sort and identify genes and proteins uniquely or preferentially expressed in embryonic OHCs
or IHCs. We will then functionally test some of these genes for roles in OHC- and IHC-specific development.
These approaches offer for the first time the means to elucidate how cochlear OHCs and IHCs differentiate
their unique features. This knowledge will be critical for the development of therapies to restore hearing due to
hair cell death, as this will require the specific replacement of OHCs, IHCs or both.

## Key facts

- **NIH application ID:** 9951028
- **Project number:** 5R01DC015903-04
- **Recipient organization:** NORTHWESTERN UNIVERSITY
- **Principal Investigator:** Jaime Garcia-Anoveros
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $634,782
- **Award type:** 5
- **Project period:** 2017-07-15 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9951028

## Citation

> US National Institutes of Health, RePORTER application 9951028, Outer and Inner Hair Cell Development (5R01DC015903-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9951028. Licensed CC0.

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