# Pluripotent Stem Cell and Organoid Core

> **NIH NIH P30** · CINCINNATI CHILDRENS HOSP MED CTR · 2020 · $138,796

## Abstract

PROJECT SUMMARY 
The overall goal of the Pluripotent Stem Cell and Organoid Core of the Digestive Health Center (DHC) is 
to provide new, cutting edge technologies that are focused on digestive disease research and enable the use 
of tissue organoids to model human disease. The Core pursues this goal with four complementary aims. In the 
first aim “to provide DHC investigators with quality tested PSCs and PSC-derived GI tissues,” the Core makes 
available to users quality-tested human pluripotent stem cells (PSCs) and PSC-derived intestinal and gastric 
organoids. These are novel 3D-miniature organs that enable studies related to physiology and pathobiology 
relevant to humans. In the second aim “to generate biopsy-derived human enteroids for DHC investigators,” 
the Core also provides investigators with the opportunity to generate gastric, intestinal, and colonic “enteroids” 
from healthy or diseased subjects. In the third aim “to derive and quality test disease-specific induced PSC,” 
the Core applies well-established transfection, culture, and phenotyping protocols to generate inducible PSCs 
(iPSCs) from normal and diseased subjects to facilitate studies of pathogenesis of disease, drug screening, 
etc. To be able to track and visualize these cells in experimental assays, the Core also provide PSC-editing 
and screening services to establish novel cell lines based on investigators' needs. And in the fourth aim “to 
sponsor training courses and workshops on PSC and organoid technology,” the Core holds regular sessions 
on basic and advanced techniques for PSC culture, generation of iPSCs, and generation and use of human 
digestive tissue organoids. These novel technologies empower DHC investigators to study mechanisms of 
disease using multi-cellular experimental systems that have direct “lineage” to normal and diseased human 
tissues (including at different stages of maturation). The delivery of services is streamlined and centralized, 
and positions investigators to explore new collaborative projects.

## Key facts

- **NIH application ID:** 9951041
- **Project number:** 5P30DK078392-14
- **Recipient organization:** CINCINNATI CHILDRENS HOSP MED CTR
- **Principal Investigator:** James M Wells
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $138,796
- **Award type:** 5
- **Project period:** 2020-06-01 → 2022-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9951041

## Citation

> US National Institutes of Health, RePORTER application 9951041, Pluripotent Stem Cell and Organoid Core (5P30DK078392-14). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9951041. Licensed CC0.

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