# Core A- Proteomics

> **NIH NIH P30** · JOHNS HOPKINS UNIVERSITY · 2020 · $137,930

## Abstract

Project Summary: Core A
 The DDBTRCC Proteomics Core A is coupled to the Johns Hopkins University (JHU) School of Medicine
Mass Spectrometry and Proteomics Facility and provides DDBTRC Center investigators faster access to the
services of the JHU Core, a designated Core Proteomics Specialist with whom to work, a specific
biostatistician/bioinformatician for post identification/quantification data analysis, all at a reduced cost. The
Proteomics Core uses mass spectrometry coupled to multi-dimensional separations by column
chromatography or gel electrophoresis to identify, quantify or characterize proteins and their post-translational
modifications, expressed in well characterized protein fractions from cells, tissues or body fluids. Techniques
such as isobaric mass tag labeling (TMT, tandem mass tags, or iTRAQ, isobaric tag for relative and absolute
quantitation) and stable isotope labeling of amino acids in cell culture (SILAC), as well as non-labeling methods
(MuDPIT, multi-dimensional protein identification technology) are available for quantifying relative differences
in protein expression and post-translational modifications, such as acetylation, AMPylation, citrullination,
glycosylation, phosphorylation, nitrosation, proline hydroxylation, ubiquitination and novel cleavage sites. For
determining the amount of specific proteins, the Core offers recently established targeted proteomic methods,
such as Absolute Quantification (AQUA) and Protein Standard Absolute Quantification (PSAQ) methods. In
addition, the Core offers high resolution mass and fragmentation analysis of intact proteins, a "top down"
approach to characterize proteins and their modifications. All Core services include pre- and post-analysis
consultation on sample preparation; a Core Proteomic Specialist assigned to receive and analyze the samples;
proteolytic digestion, peptide extraction, labeling and fractionation; nano-liquid chromatography separation
coupled to tandem mass spectrometry analysis; database searching, data analysis and interpretation,
compiled results reports; and educational programs through core presentations and technical workshops. A
biostatician/bioinformatician is available when more in depth statistical and ontological analyses of the
proteomic results is needed. For DDBTRCC investigators who wish to perform their own analysis the Core
provides access to proprietary software and equipment, and Core workshops are offered several times a year
on operating the Core MALDI mass spectrometer. 18 current Members, 6 Associate Members and a total of 30
Core Center investigators from our entire funding period have used the Proteomics Core and 51 manuscripts
are attributed to this Core.

## Key facts

- **NIH application ID:** 9951048
- **Project number:** 5P30DK089502-10
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **Principal Investigator:** Robert N Cole
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $137,930
- **Award type:** 5
- **Project period:** — → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9951048

## Citation

> US National Institutes of Health, RePORTER application 9951048, Core A- Proteomics (5P30DK089502-10). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9951048. Licensed CC0.

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