# Core C- Physiology

> **NIH NIH P30** · JOHNS HOPKINS UNIVERSITY · 2020 · $251,610

## Abstract

Integrated Physiology Core C: Project Summary
 The Integrated Physiology Core is a multi-service resource that provides Center Investigators and their
laboratories the tools and advice needed to establish and study mouse (including transgenic and knockout)
and human (i.e. 3D organoid cultures) models of gastrointestinal disease. Since the inception of our Center,
this Core has expanded its use by the Research Base and become a highly cost effective and efficient
resource for histology, genotyping, and physiology services. This Core also provides the Research Base with
the training and resources to utilize human intestinal stem cell technology for their studies. The use of human
intestinal stem cell derived enteroids/colonoids has rapidly evolved and is becoming a premier model for the
study of human GI physiology and pathophysiology. During the initial funding period, the Core was used by 46
Core Center investigators (>70% utilized multiple services), resulting in 65 published manuscripts, and has
been used by 7 out of 11 funded P/F projects. The services offered include 1) Advice on establishing and
maintaining mouse colonies, including how to breed onto uniform backgrounds; 2) Genotyping, which includes
developing and optimizing primers; 3) Histological services, which include tissue fixation and processing,
embedding, sectioning (including cryosectioning), histopathological staining (e.g. H&E, PAS, Sirius Red), and
tissue archiving. A tissue bank of H&E slides of GI organs of the mouse models studied by our Research Base
are made available for other Core members to use for preliminary studies; 4) Histopathology consultation by an
expert pathologist for characterization of mouse GI disease models; 5) Metabolic cages are available for
metabolic balance studies, including blood, urine, stool analysis; 6) Ussing chamber/voltage clamp technology
for measuring active ion transport and tight junction permeability and permselectivity;7) Multiplex ELISA for
simultaneous measurement of ~100 analytes (e.g. cytokines, chemokines, growth factors) from a small volume
(<100μl) sample of serum, tissue, or culture media; as well as 8) Training in establishing, maintaining, and
using mouse and human enteroids as well as provision of growth factor conditioned media necessary for long-
term culture.

## Key facts

- **NIH application ID:** 9951050
- **Project number:** 5P30DK089502-10
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **Principal Investigator:** Nicholas Constantine Zachos
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $251,610
- **Award type:** 5
- **Project period:** — → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9951050

## Citation

> US National Institutes of Health, RePORTER application 9951050, Core C- Physiology (5P30DK089502-10). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9951050. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*