# Core D - Protein Production and Interaction

> **NIH NIH P01** · VANDERBILT UNIVERSITY MEDICAL CENTER · 2020 · $122,297

## Abstract

ABSTRACT: PROTEIN PRODUCTION AND INTERACTION CORE (CORE D)
The Protein Production and Interaction Core (Core D) will be led by W. Sean Davidson and will be located in
his laboratory at the University of Cincinnati. His lab has extensive and documented experience in all proposed
Core services. The Core is designed to provide a consistent and cost-effective set of high quality protein and
lipoprotein reagents to PPG investigators in furtherance of the goals of each Project. Core D also provides
evaluations of protein:protein interactions by developing and implementing state-of-the-art cross-linking
approaches for application across the PPG projects as well as continued technology development with the aim
of improving the resolution of structural determinations. The Core will be heavily used by each of the Projects.
The goals of Core D are:
1. To express, purify and distribute recombinant apolipoproteins and their mutants produced in bacteria.
2. To express and distribute PON1, CETP, apoA-II and ABCA1 and their mutants produced in eukaryotic
cells.
3. To isolate, characterize and distribute lipoproteins and apolipoproteins isolated from human plasma.
4. To generate, characterize and distribute reconstituted HDL lipoproteins.
5. To perform chemical cross-linking analyses on individual proteins, enzymes, transfer proteins and
 lipoprotein complexes in synergy with the HDL Quantitation Core (Core C).
6. To develop innovative new cross-linking approaches aimed at increasing protein structure resolution.

## Key facts

- **NIH application ID:** 9951097
- **Project number:** 5P01HL128203-05
- **Recipient organization:** VANDERBILT UNIVERSITY MEDICAL CENTER
- **Principal Investigator:** W Sean Davidson
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $122,297
- **Award type:** 5
- **Project period:** — → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9951097

## Citation

> US National Institutes of Health, RePORTER application 9951097, Core D - Protein Production and Interaction (5P01HL128203-05). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9951097. Licensed CC0.

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