# Physiology Core

> **NIH NIH P30** · UT SOUTHWESTERN MEDICAL CENTER · 2020 · $261,687

## Abstract

PROJECT SUMMARY/ABSTRACT
Rodents are important tools for studying kidney function and diseases. However, expertise and equipment for
physiological analysis of kidney function in mice are not readily available to many investigators in the renal
research community, and even less so outside the renal community. Furthermore, production of mutant mice
has allowed many investigators outside the renal community to uncover unexpected renal phenotypes and
venture into renal research. The objective of the Physiology Core is to assist investigators with a wide range of
physiological techniques to study kidney physiology and diseases at the cellular, organ, and importantly, whole
animal level. This Core serves the three major themes of the O'Brien Center of Renal Development and Genetics,
Physiology and Pathophysiology, Chronic Kidney Disease and tis Complications.
To achieve these objectives, the Core will provide specific assistances:
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
Whole animal metabolic and clearance studies
Measurement of blood pressure by tail cuff or telemetry
Measurements of serum and urine creatinine and citrate
Measurement of serum and urine electrolytes and chemistry
Serum or urine soluble Klotho by our own immunoprecipitation-immunoblot
Electrolytes in nanolitre volume using ion-selective electrodes
Isolation of individual tubules for real-time PCR and immuno-fluorescent staining
In vitro microperfusion of individually isolated tubules
Electro-physiological studies
Participation of an exploratory Klotho ELISA an Alpha LISA
1 to 5 are offered as services with nominal non-profit cost recovery charges. 6 to 9 are offered as collaborations
with mutual agreement between Investigators and Core B Directors with clearly defined deliverables, no cost,
but with joint authorships. 10 is a new exploratory techniques where investigators can work with our Core
Directors to co-develop these new assays by contributing samples, scientific, or technical input. There will be no
charge and any resultant paper (no guaranteed deliverables) will be co-authored. Surveillance is in place to
evaluate efficiency and to maintain quality control of services provided.
The Core will also provide education and training to the investigators and their staff and trainees. The Core
Directors have a track record of providing assistance and training to visiting scientists locally and nationally.
The Physiology Core Kidney contributes to the understanding of themes of the O'Brien Center in kidney
development and genetic, renal physiology and pathophysiology, and chronic kidney disease, and our long-term
goal of improving the diagnosis and treatment of kidney diseases.

## Key facts

- **NIH application ID:** 9954061
- **Project number:** 5P30DK079328-14
- **Recipient organization:** UT SOUTHWESTERN MEDICAL CENTER
- **Principal Investigator:** Orson W Moe
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $261,687
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9954061

## Citation

> US National Institutes of Health, RePORTER application 9954061, Physiology Core (5P30DK079328-14). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9954061. Licensed CC0.

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