# Protein Production and Protein Interaction Core

> **NIH NIH P01** · WASHINGTON UNIVERSITY · 2020 · $614,750

## Abstract

Filoviruses are CDC category A priority pathogens that can subvert host immune systems to facilitate viral 
replication, resulting in human case fatality rates as high as 90%. The Ebola virus (EBOV) RNA-dependent RNA 
polymerase (RDRP) complex is critical for replication. However, the mechanisms regulating viral RNA synthesis 
or the factors that modify viral replication are not understood. The Protein Production and Protein Interaction 
Core (Core B) will provide critical reagents and support for the overall scientific mission and facilitate the 
characterization of viral-viral and viral-host interactions. Specifically, Core B will provide a unique set of 
standardized reagents and validation data that will be disseminated amongst the Projects and Cores. Core B 
has prioritized experiments in order to ensure that reagents will be readily available for use by the Projects and 
Cores. Core B will generate milligram quantities of highly purified recombinant viral RDRP proteins that will be 
used for evaluation of strain similarities/differences and for both monoclonal antibody development and synthetic 
antibody generation. Core B will also produce recombinant novel host factors identified by Projects 1 and 3 that 
will be used to validate and characterize interfaces between the EBOV RDRP complex and novel host factors.

## Key facts

- **NIH application ID:** 9955172
- **Project number:** 5P01AI120943-05
- **Recipient organization:** WASHINGTON UNIVERSITY
- **Principal Investigator:** Daisy W Leung
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $614,750
- **Award type:** 5
- **Project period:** 2016-07-07 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9955172

## Citation

> US National Institutes of Health, RePORTER application 9955172, Protein Production and Protein Interaction Core (5P01AI120943-05). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/9955172. Licensed CC0.

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