# Molecular Biology and Genetics Core

> **NIH NIH P01** · UNIVERSITY OF ARIZONA · 2020 · $252,420

## Abstract

ABSTRACT:
This Molecular Biology & Genetics Core (Core B) will provide essential, cutting edge, molecular biology and
genetics expertise to facilitate the translational impact of this highly integrated PPG focused on ventilator-
induced lung injury (VILI) / acute respiratory distress syndrome (ARDS). PPG Core B will be responsible for
the generation of promoter luciferase reporter constructs, as well as modified promoter luciferase reporter
constructs (serially deleted or point mutated). Core B will also generate expression DNA plasmids for key
proteins studies in all three Projects for different over-expression systems including the E. Coli. system (for
Core D to generate recombinant protein), mammalian cell system (for projects to study protein function in
endothelial cells or murine lung delivery). We will also perform site-directed mutagenesis (SDM) on these
expression constructs to introduce mutations representing prioritized single nucleotide polymorphisms (SNPs)
or post-translational modifications (PTMs) of interest. Core B will also provide epigenetic analysis on DNA
methylation and acetylation with routine molecular biology methods. As a validation step, Core B will perform
DNA-protein interaction characterizations utilizing electrophoretic mobility shift assay (EMSA) and chromatin
immunoprecipitation (ChIP). In addition, Core B will provide Project investigators with a well-characterized
ARDS biobank (i.e. DNA from European descent and African descent subjects), a complete list of selected
SNPs for each PPG candidate gene, mid-throughput genotyping services, and data analysis tools to test the
association between PPG selected SNPs and susceptibility and severity of VILI/ARDS. Thus, overall Core B
will utilize these technologies to provide a molecular biology and genetics study platform for all three Projects
and generate novel information on the genes targeted for investigation in this PPG. All three Projects and
Core D will utilize this centralized core for the molecular biology and genetics analyses planned in this PPG.

## Key facts

- **NIH application ID:** 9959501
- **Project number:** 5P01HL134610-03
- **Recipient organization:** UNIVERSITY OF ARIZONA
- **Principal Investigator:** Joe G. N. Garcia
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $252,420
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9959501

## Citation

> US National Institutes of Health, RePORTER application 9959501, Molecular Biology and Genetics Core (5P01HL134610-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9959501. Licensed CC0.

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