# BioImaging and BioTechnology Implementation Core

> **NIH NIH P01** · UNIVERSITY OF SOUTH ALABAMA · 2020 · $227,646

## Abstract

CORE SUMMARY
Core D will implement hyperspectral imaging technologies, image analysis approaches, and mathematical
modeling to answer questions concerning the time course and spatial spread of cellular signals and
subsequent regulation of endothelial function. Core D will support all PROJECTS by providing spectral analysis
of the molecular composition of tissues, enabling quantitative imaging of localized intracellular signals and
implementing novel image processing, data analysis, and mathematical modeling approaches.
Service component: Core D will provide capabilities for next-generation imaging, image analysis, quantitative
data extraction, mathematical modeling, and data storage and retrieval to support all projects and cores. In
specific, Core D will 1) provide expertise and assistance in implementing next-generation 5-dimensional
imaging (x,y,z,t,λ) approaches; 2) develop customized analysis approaches for extracting localized signaling
information from 5-dimensional image data; 3) assist in creating mathematical models of signaling pathways to
aid in hypothesis testing and generation; and 4) manage a central repository for image and modeling data
storage that will facilitate cross-project collaboration and data mining.
Academic component: The investigators of Core D will continue to develop new technologies for next-
generation hyperpsectral, high-speed microscopy. As these microscope systems are implemented, Core D will
provide access to and training on the new imaging technologies. During the upcoming cycle, Core D will focus
on implementing several high-speed hyperspectral imaging technologies that are currently in the late stages of
prototype development. Access to these technologies will enable project investigators to take the next step in
studying pulmonary vasculature on a cellular and biosystems level (for example, simultaneous subcellular
measurements of Ca2+-cGMP-NO, or assessing cAMP in whole tissue constructs and intravitally).
Synergy with projects and scientific cores: Core D will serve as a hub for project synergy by implementing
cross-project quantitative imaging, analysis, and modeling approaches and by ensuring that all image and
modeling data is archived and maintained in a central database (repository). The quantitative nature of 5D
imaging and the ability to access data from experiments across multiple projects will further build synergy. For
example, we can quantitatively assess whether Ca2+ influx through T-type channels in response to a 20 second
depolarization is sufficient to modulate sAC and AC6 activity at designated subcellular locations and to effect
changes in cAMP and endothelial barrier integrity. Thus, the quantitative nature of Core D provides a
framework for interweaving imaging and modeling studies performed by all projects.

## Key facts

- **NIH application ID:** 9962988
- **Project number:** 5P01HL066299-18
- **Recipient organization:** UNIVERSITY OF SOUTH ALABAMA
- **Principal Investigator:** Silas Josiah Leavesley
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $227,646
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9962988

## Citation

> US National Institutes of Health, RePORTER application 9962988, BioImaging and BioTechnology Implementation Core (5P01HL066299-18). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9962988. Licensed CC0.

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