# Biosensor Development and Expression

> **NIH NIH P30** · INDIANA UNIVERSITY INDIANAPOLIS · 2020 · $205,161

## Abstract

Abstract (Biosensor Development and Expression)
The Biosensor Development and Expression Core will develop novel resources for the renal
research community designed to enhance the research potential of the intravital light
microscopy core facilities offered by the Indiana University O'Brien Center of Excellence. Using
the experience and reagents developed during the prior funding period as a springboard, the
aims of the core are to develop the next generation of biosensors, optimized gene delivery
systems, and develop unique transgenic animal models for renal research. The first aim is the
continued development of genetically encoded biosensor probes optimized for intravital
microscopy (IVM). To facilitate the targeted delivery of these probes, the second aim will
develop a RGD modified Gateway-acceptor recombinant adenovirus with tet suppressible
transgene expression. This reagent will be optimized for gene delivery to the kidney
parenchyma and is designed to permit rapid and safe production of recombinant adenovirus.
The third aim is to produce a transgenic Munich-Wistar-Frömter rat that constitutively expresses
a tet-off transactivator that will allow tight control of the expression of the adenovirus vectors
created in aim 2. The final aim is to generate transgenic rats with tet-off expression control of
optical highlighters targeted to podocyte or mesangial cells, which will enable tracking
experiments in response to glomerular injury, sepsis, ischemia, diabetes and hypertension. We
anticipate widespread utilization the services and reagents developed by our Core facility, and
strive to catalyze and accelerate scientific inquiry in renal research.

## Key facts

- **NIH application ID:** 9963205
- **Project number:** 5P30DK079312-14
- **Recipient organization:** INDIANA UNIVERSITY INDIANAPOLIS
- **Principal Investigator:** ROBERT L BACALLAO
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $205,161
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9963205

## Citation

> US National Institutes of Health, RePORTER application 9963205, Biosensor Development and Expression (5P30DK079312-14). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9963205. Licensed CC0.

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