# Tools For High-Throughput High-Resolution Three Dimensional Electron Microscopy

> **NIH NIH R01** · FLORIDA STATE UNIVERSITY · 2020 · $321,288

## Abstract

Summary
Three-dimensional electron microscopy (3DEM) is a set of techniques for determining the structures of
large proteins and protein complexes. It has been shown that cryogenic EM (cryo-EM) has the ability to
determine structures to high enough resolution to unambiguously place side chains in the EM density.
Though cryo-EM is increasingly reaching near-atomic resolution, a number of hurdles are present in
order to achieve side-chain level resolution structure determination of low-symmetry and no symmetry
complexes. Here we propose to develop tool to facilitate high-throughput high-resolution 3DEM. In the
first aim, we propose to develop tools to enable automated specimen preparation and screening, and
enable a new tools for direct electron detection. In the second aim, we propose to develop tools for
tomographic data collection and processing including exploring new modalities for image acquisition
and for developing an infrastructure for processing tomographic subvolumes. This research will benefit
human health by facilitating the determination of protein structures that are relevant for human disease.

## Key facts

- **NIH application ID:** 9963262
- **Project number:** 5R01GM108753-05
- **Recipient organization:** FLORIDA STATE UNIVERSITY
- **Principal Investigator:** SCOTT M STAGG
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $321,288
- **Award type:** 5
- **Project period:** 2015-09-30 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9963262

## Citation

> US National Institutes of Health, RePORTER application 9963262, Tools For High-Throughput High-Resolution Three Dimensional Electron Microscopy (5R01GM108753-05). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9963262. Licensed CC0.

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