# Core C: Molecular Analysis

> **NIH NIH P01** · UNIV OF ARKANSAS FOR MED SCIS · 2020 · $203,440

## Abstract

Summary
The purpose of Core C is to provide, on request, molecular analysis services to the investigators in this
Program Project. The services will include western immunoblot, quantitative (real-time) reverse-transcriptase
polymerase chain reaction (qRT-PCR) analyses, ELISAs, and 2D-gel proteomic preparation. In addition, other
forms of macromolecular analysis will be developed as the need arises. The tissue to be analyzed will be from
human and rodent brain and from nematode and cell cultures. All three Projects will utilize the services of
Core C. A key aspect of the Core will be provision of expertise in time-consuming steps, such as antibody
selection and optimization of PCR parameters. In addition, standardization across projects will help to achieve
valid comparisons and improve efficiency. Results from analyses performed in this Core will be provided to
Project Leaders primarily as raw data, including appropriate controls to document sample integrity and
equipment function. Data for some analyses will be supplemented by routine extrapolation, and Core
personnel will consult with Project Leaders in developing sophisticated interpretation of data and experimental
parameters. Residual samples and processed specimens, such as tissue sections, will be returned to the
respective Project Leader for archiving. Records of services performed and quantitative data from the Core
will be archived in the Core's computer database, under protection of state-of-the-art firewalls and data
backup. Achieving the goals of this Core will provide efficiency and standardization of methods for data
acquisition and analyses across projects and will help ensure effective collection and dissemination of data for
publication.

## Key facts

- **NIH application ID:** 9964633
- **Project number:** 5P01AG012411-21
- **Recipient organization:** UNIV OF ARKANSAS FOR MED SCIS
- **Principal Investigator:** Steven W Barger
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $203,440
- **Award type:** 5
- **Project period:** 1997-08-01 → 2023-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9964633

## Citation

> US National Institutes of Health, RePORTER application 9964633, Core C: Molecular Analysis (5P01AG012411-21). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9964633. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*