# INVESTIGATING NAPDH OXIDASES AND REDOX SIGNALING IN LUNG CANCER TUMORIGENICITY

> **NIH NIH R01** · WASHINGTON UNIVERSITY · 2020 · $554,382

## Abstract

Project Summary
Lung cancer results in the largest number of cancer-related deaths in the United States. Non-small cell lung
cancer (NSCLC) patient tumors overexpress NADPH oxidases that produce intracellular hydrogen peroxide
(H2O2) and play an important role in lung cancer tumorigenicity. To date, our preliminary results demonstrate
that NOX activity is functionally important for NCSLC transformation, escape from anoikis, phosphorylation
signaling, and redox regulation of cysteines in several novel intracellular proteins. The fundamental goal of this
proposal is to study the molecular details of NOX-dependent redox signaling in lung cancer biology. Aim 1 of
the proposal will characterize the functional role of NOX isoforms overexpressed in lung patient tumors in lung
cancer tumorigenicity. Aim 2 will examine the regulatory crosstalk between phosphorylation signaling and NOX
activity by profiling NOX-dependent kinase activity. Aim 3 will quantitatively map the cysteine targets of NOX-
derived H2O2 in NSCLC upon NOX inhibition. Investigating the role of NOX enzymes and redox signaling in
lung cancer will shed light on orthogonal control mechanisms regulating cancer cell growth, transformation and
evasion of anoikis. There is a strong link between RAS mutations, overexpression of NOX and increased ROS
production. Therefore, investigating redox signaling in NSCLC may identify new regulators and therapeutic
targets for intervention in lung cancer, especially RAS-driven tumors with no clear therapeutic option.

## Key facts

- **NIH application ID:** 9964687
- **Project number:** 5R01CA200893-05
- **Recipient organization:** WASHINGTON UNIVERSITY
- **Principal Investigator:** Jason M. Held
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $554,382
- **Award type:** 5
- **Project period:** 2016-07-01 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9964687

## Citation

> US National Institutes of Health, RePORTER application 9964687, INVESTIGATING NAPDH OXIDASES AND REDOX SIGNALING IN LUNG CANCER TUMORIGENICITY (5R01CA200893-05). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/9964687. Licensed CC0.

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