# Flow Cytometry Core

> **NIH NIH P20** · RHODE ISLAND HOSPITAL · 2020 · $245,789

## Abstract

Flow Cytometry Core
Abstract
The Flow Cytometry Core will provide expertise in stem cell separations, cell sorting and cell phenotyping
analysis. The Core will be co-directed by Dr. Loren Fast and Mr. Mark Dooner. Mr. Dooner is an experienced
flow cytometrist with sorting and analysis of neural and hematopoietic stem cells, and will oversee the daily
operation including cell sorting and analysis. Dr. Fast will serve as an advisor and resource to the investigators
for experimental design, data interpretation and manuscript preparation. During Phase I, instrument upgrades
created a five laser cell sorter that is unique in the Rhode Island research community. This Core features an
Influx high speed cell sorter (BD Biosciences). This sorter is capable of carrying out stem cell separations,
single cell deposition, and simultaneous six-way sorting and is housed within a biosafety cabinet. This Core is
housed in a newly constructed and dedicated suite. The facility is equipped with a LSRII flow cytometer (BD
Biosciences) with FACS Diva software for cell cycle analysis and routine flow cytometry analysis. Investigators
will work with the Core and prepare their cell populations for sorting or analysis. The Core will serve all
projects in this COBRE carrying out both separations and analysis work free of charge. Non-COBRE
investigators are making use of the Core on a fee for service basis however COBRE Projects will be given
priority. The Core remains dedicated to the development of new separation techniques and the enhancement
of known neural and hematopoietic stem cell populations.

## Key facts

- **NIH application ID:** 9965979
- **Project number:** 5P20GM119943-04
- **Recipient organization:** RHODE ISLAND HOSPITAL
- **Principal Investigator:** MARK S DOONER
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $245,789
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9965979

## Citation

> US National Institutes of Health, RePORTER application 9965979, Flow Cytometry Core (5P20GM119943-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9965979. Licensed CC0.

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