# Proteomics Core Defining the unique properties of the distinct signaling machinery used by the TCR

> **NIH NIH P01** · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · 2020 · $141,803

## Abstract

PROTEOMICS CORE SUMMARY/ABSTRACT
A comprehensive molecular understanding of T cell receptor proximal signaling and the biochemical networks
regulated by Ras exchange factors in peripheral T cell signaling requires both a global analysis of
phosphorylation events and protein interactions across the entire proteome of a cell as they occur in either the
basal or stimulated T cell state. The proteomic core will make these cutting-edge, quantitative capabilities
available to the investigators of this proposal. The Proteomics Core will provide essential expertise for the
timely analysis of the protein composition and post-translational modifications for both projects described in
this proposal. The proteomic core will provide identification and relative quantitation of the protein composition
and post-translational modification state of proteins using cutting-edge LC/MS techniques using a recently
acquired Q Exactive mass spectrometer. Dr. Arthur Salomon, who has been using LC/MS technology to study
TCR signaling pathways for more than a decade, brings his expertise to the Program and serves as
Proteomics Core facility Director. The core will identify tyrosine as well as serine threonine phosphorylation
sites from SILAC and label-free samples from T cell lines, primary mouse T cells, and in vitro kinase reactions
from Project #1 (Weiss, PI) and protein-protein interactors from Project #2 (Roose PI). It will also perform
statistical evaluation and interpretation of phosphorylation sites from cell studies.

## Key facts

- **NIH application ID:** 9966854
- **Project number:** 5P01AI091580-10
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- **Principal Investigator:** ARTHUR Robert SALOMON
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $141,803
- **Award type:** 5
- **Project period:** 2011-07-15 → 2022-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9966854

## Citation

> US National Institutes of Health, RePORTER application 9966854, Proteomics Core Defining the unique properties of the distinct signaling machinery used by the TCR (5P01AI091580-10). Retrieved via AI Analytics 2026-06-01 from https://api.ai-analytics.org/grant/nih/9966854. Licensed CC0.

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