# Histopathology Core

> **NIH NIH P01** · BAYLOR COLLEGE OF MEDICINE · 2020 · $149,835

## Abstract

Abstract: 
Renal disease in HIV-infected patients has evolved over the 3 decades since the outset of the 
infection. Whereas HIV-associated nephropathy (HIVAN) manifesting collapsing 
glomerulopathy, tubular microcysts and endothelial tubuloreticular inclusions predominated 
early in the epidemic, the incidence of HIVAN has declined following the introduction of anti- 
retroviral therapy. Despite this trend, the incidence of ESRD has remained unchanged, 
reflecting the development of other histopathology. This proposal will explore the long-term 
consequences of HIV infection in the kidney beyond HIVAN. The role of Core B will be to 
provide technical and interpretative support in light microscopy, immunohistochemistry, 
immunofluorescence and electron microscopy in mouse and human kidney tissues for all 
projects. For Specific Aim 1, archived kidney biopsy tissue from HIV positive patients will be 
selected based on histopathologic diagnosis for investigation of inflammatory pathways 
identified in the in vitro models using immunohistochemistry and immunofluorescence. In 
Specific Aim 2, renal pathology in Tg26 mice with different intestinal microbiome will be 
evaluated and immunostaining performed to quantify immune cell infiltrates. For Specific Aim 3, 
the hypothesis that chronic HIV infection promotes comorbid disease though enhanced pro- 
inflammatory response via SIRT-1-regulated NF-kB and STAT3 acetylation and mitochondrial 
injury will be investigated in murine kidneys and validated in human kidney tissues. Core B will 
collaborate with the Clinical Core to identify appropriate kidney biopsy samples from the Core C 
repository or from anonymized archived samples in the Columbia biopsy database as needed.

## Key facts

- **NIH application ID:** 9966958
- **Project number:** 5P01DK056492-20
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **Principal Investigator:** VIVETTE D'AGATI
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $149,835
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9966958

## Citation

> US National Institutes of Health, RePORTER application 9966958, Histopathology Core (5P01DK056492-20). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9966958. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*