# TR&D2-DNP

> **NIH NIH P41** · FLORIDA STATE UNIVERSITY · 2020 · $186,408

## Abstract

TR&D2: Biomedical Research Utilizing Dynamic Nuclear Polarization
Project Summary/Abstract
The development of dynamic nuclear polarization (DNP) to increase the sensitivity of NMR by 1-2 orders of
magnitude is profoundly impacting the field of NMR. Early results demonstrating the utility of DNP when
combined with magic angle spinning (MAS) solid state NMR (ssNMR) for biomolecular studies provide impetus
for further developing DNP technology to support biomedical research applications. This TR&D addresses
challenges the NMR community faces in making DNP a truly orthogonal, enabling technology for biomolecular
ssNMR through three specific aims: 1) develop DNP MAS ssNMR probes with flexible electronic architecture,
fast MAS, and extended VT Range; 2) develop a biradical toolbox for targeted DNP enhancement of specific
protein classes; and 3) optimize DNP MAS ssNMR approaches for biomolecular structure determination. The
goal of these aims is to provide a holistic approach to maximizing the promise of DNP through hardware
development, synthesis of needed chemical reagents, optimization of sample preparation strategies, and
developing the experimental framework for biomolecular ssNMR in the age of DNP. Advances in all these
areas must be pursued to gain the needed sensitivity and resolution for leading edge biomedical research
applications and to transition DNP MAS ssNMR to a technique that is more widely accessible to the structural
biology community as a whole. Through partnering with six Driving Biomedical Research projects, we will
rapidly develop and deploy technology in this TR&D to address biomedical research problems in the areas of
membrane protein structure determination, amyloid biogenesis in bacterial biofilms and in neurodenerative
diseases, protein assembly in viruses, and enzyme catalysis.

## Key facts

- **NIH application ID:** 9967075
- **Project number:** 5P41GM122698-04
- **Recipient organization:** FLORIDA STATE UNIVERSITY
- **Principal Investigator:** Joanna R Long
- **Activity code:** P41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $186,408
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9967075

## Citation

> US National Institutes of Health, RePORTER application 9967075, TR&D2-DNP (5P41GM122698-04). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9967075. Licensed CC0.

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