# Metabolic and Molecular Laboratory Core

> **NIH NIH P01** · NORTHWESTERN UNIVERSITY · 2020 · $323,800

## Abstract

The main function of the Core is to provide state-of-the-art analytic methods to advance the proposed
research in all three projects of the Program Project. We will provide reliable measurements and analyses of
blood glucoregulatory, appetitive, and circadian hormones and other metabolite from human subjects who are
affected by aging and/or sleep duration and quality in Projects 1 and 2. Assays will include leptin, ghrelin, gastric
inhibitory polypeptide (GIP), C-peptide, cortisol, melatonin (from blood and saliva), 6-sulfatoxymelatonin (MTS6
in urine), glucose, insulin, lactate, pyruvate, free fatty acids (FFA), triglycerides, LDL, HDL, HbA1c, and NAD+.
We will all also provide metabolic phenotyping methods to study tissue-specific and cellular metabolic flux in
both the animal tissues and human blood samples and biopsies. The analytical procedures include high-
performance liquid chromatography and mass spectrometry for metabolite analyses, cellular bioenergetics, real
time monitoring of cellular redox state, biochemical analysis of sirtuin activity, gene expression analysis using
quantitative PCR methods, as well as transcriptome analyses using next-generation sequencing for animal
models in Project 3.
 All analytical methods have been validated and operational in our laboratory. The Metabolic and
Molecular Core will provide critical support for the implementation of all Projects, explore potential use of novel
analytical techniques, and will be closely integrated with the Analysis Core to ensure timely and safe archival of
laboratory data.

## Key facts

- **NIH application ID:** 9969248
- **Project number:** 5P01AG011412-21
- **Recipient organization:** NORTHWESTERN UNIVERSITY
- **Principal Investigator:** Joseph Bass
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $323,800
- **Award type:** 5
- **Project period:** — → —

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9969248

## Citation

> US National Institutes of Health, RePORTER application 9969248, Metabolic and Molecular Laboratory Core (5P01AG011412-21). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9969248. Licensed CC0.

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