# Extramedullary Hematopoiesis and the Splenic Niche

> **NIH NIH R01** · UNIVERSITY OF FLORIDA · 2020 · $274,500

## Abstract

In this proposal we show the preliminary development of a novel intravital methods to concurrently visualize
both splenic colony formation and bone marrow reconstitution by fluorescently tagged donor HSC/HPC
populations. Our tibia window model allows direct visualization of early marrow engraftment dynamics of
fluorescently tagged HSC/HPC populations in individual living mice for up to three weeks. The intravital CFU-
Spleen model currently allows 1-2 concurrent observations of the extramedullary splenic microenvironments
and bone marrow longitudinally in individual transplant recipients. The primary goal of this SHINE-II
proposal is to improve the intravital CFU-Spleen model to increase the number and resolution of the spleen
observations to better match the tibia window model. The ability to observe both marrow and extramedullary
donor HSC/HPC engraftment and niche utilization dynamics in living recipients over multiple days/weeks post
transplant will significantly enhance our understanding of transplant biology.

## Key facts

- **NIH application ID:** 9969482
- **Project number:** 5R01DK121117-02
- **Recipient organization:** UNIVERSITY OF FLORIDA
- **Principal Investigator:** Edward W Scott
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $274,500
- **Award type:** 5
- **Project period:** 2019-07-03 → 2022-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9969482

## Citation

> US National Institutes of Health, RePORTER application 9969482, Extramedullary Hematopoiesis and the Splenic Niche (5R01DK121117-02). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/9969482. Licensed CC0.

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