# Flow Cytometry

> **NIH NIH P30** · UNIVERSITY OF ARIZONA · 2020 · $84,066

## Abstract

Project Summary / Abstract: Flow Cytometry Shared Resource (FCSR) 
The goal of the Flow Cytometry Shared Resource (FCSR), which has served investigators of the University of 
Arizona Cancer Center (UACC) for more than 25 years, is to offer UACC Members efficient and reliable flow 
cytometric services with a high standard of quality control and to do so in a cost-effective manner. The FCSR 
provides the knowledge and assistance necessary to design experiments, develop new assays, perform flow 
cytometric functions, and interpret data. In 2004, this service merged with Arizona Research Laboratories 
(ARL) flow cytometry service, making the UACC FCSR the only University of Arizona research flow cytometry 
shared resource, and the FCSR also houses the only cell sorter on the UA Tucson campus. The partnership 
with the ARL has resulted in strong institutional commitment to the FCSR enabling the purchase of new 
equipment and support of service contracts, as well as maximizing the utilization of resources including 
staffing. The FCSR provides the following services: 1) flow cytometric analysis; 2) high-speed aseptic cell 
sorting; 3) user-based (unassisted) flow cytometric analysis; 4) assisted flow cytometric analysis; 5) quality 
control / quality assurance; 6) data storage, management and analysis; 7) consultation in experimental design 
and data interpretation. Within these categories of service, the FCSR provides state-of-the-art analysis of cell 
surface proteins on primary human and murine cells and cultured cell lines. It offers detection of cancer 
chemotherapeutic agents that are fluorescent (e.g. doxorubicin), reporter dyes, intracellular pH and calcium 
measurement, cell cycle analysis, apoptosis, autophagy, membrane potential, intracellular detection of 
proteins, DNA ploidy analysis, cell proliferation, cytokine detection, cell sorting, and more. The FSCR also 
works to facilitate projects that require integration of multiple core resources (e.g., cell sorting followed by 
downstream genomic analysis). To inform investigators on new developments in flow cytometry techniques, 
the FCSR regularly sponsors educational and training seminars. Hands-on-training is also provided for 
students to learn antibody-based sample preparation and optimization, instrumentation operation, and data 
analysis. The FCSR is Good Laboratory Practice and Biosafety Level 2 certified.

## Key facts

- **NIH application ID:** 9969486
- **Project number:** 5P30CA023074-40
- **Recipient organization:** UNIVERSITY OF ARIZONA
- **Principal Investigator:** Yi Zeng
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $84,066
- **Award type:** 5
- **Project period:** — → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9969486

## Citation

> US National Institutes of Health, RePORTER application 9969486, Flow Cytometry (5P30CA023074-40). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/9969486. Licensed CC0.

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