# Stanford Tissue Mapping Center

> **NIH NIH U54** · STANFORD UNIVERSITY · 2020 · $1,693,777

## Abstract

ABSTRACT: Overall
The small bowel and colon are organs critical for maintaining homeostasis of the human body by
mediating nutritional absorption upon the ingestion of food. Though both organs are extensive in length,
there are known differences in function and cellular heterogeneity within different portions of each. Also,
a cross section anywhere in the bowel reveals a complex layering of components involved in absorption
and secretion, motility of gut contents, circulation, and immunity. In this submission, we propose the
establishment of the Stanford Tissue Mapping Center (TMC) to map the complexity of the the small
bowel and colon with cell-to-cell resolution in histologic sections, both along their lengths and across
multiple individuals. To accomplish this goal, we will collect tissues from brain-dead organ donors with
explicit consent for distribution among the HuBMAP consortium and open access genome data sharing
(GDS). Two sets of technologies will then be employed. Tissue samples will be subjected to single cell
(sc) ATAC-seq and scRNA-seq. These ‘omic profiles will then be spatially mapped back to histologic
sections using a highly multiplexed system of antibody-tagged target epitopes, which we call
CO-Detection by indEXing (CODEX). An integrative analysis will be performed to identify proteins
important to establishing the identity of each cell population within the tissue.
​

## Key facts

- **NIH application ID:** 9969579
- **Project number:** 5U54HG010426-03
- **Recipient organization:** STANFORD UNIVERSITY
- **Principal Investigator:** GARRY P NOLAN
- **Activity code:** U54 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $1,693,777
- **Award type:** 5
- **Project period:** 2018-09-19 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9969579

## Citation

> US National Institutes of Health, RePORTER application 9969579, Stanford Tissue Mapping Center (5U54HG010426-03). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9969579. Licensed CC0.

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