# Imaging and Cell Culture

> **NIH NIH P01** · UNIVERSITY OF ILLINOIS AT CHICAGO · 2020 · $244,790

## Abstract

Abstract
The focus of the Program Project is to investigate the molecular and signaling mechanisms of re-sealing of
adherens junctions (AJs) and restoration of lung endothelial barrier and homeostasis post-inflammatory lung
injury. These processes will be defined by means of dynamic changes in the distribution of proteins comprising
AJs including signaling molecules, assessment of their dynamics and activity at the level of lamellipodia
protrusions and AJs. Core B will provide technical support for all Projects in addressing the role of key signaling
molecules involved in lung endothelial barrier restoration as proposed including the vascular endothelial protein
tyrosine phosphatase (VE-PTP) in Project 1, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFK-
FB3) in Project 2, sphingosine-1-phosphate receptor-1 (S1PR1) in Project 3, and phospholipase D2 (PLD2) in
Project 4. This will involve advanced live-cell imaging and state-of-art image analysis to investigate (i)
distribution and dynamics of adhesion (such as VE-cadherin) and signaling proteins at AJs and in lamellipodia;
(ii) key role of PFK-FB3 and PLD2 in lamellipodia formation and dynamics and the re-sealing of AJs; (iii)
spatiotemporal activity of small RhoA GTPases Rac1, Cdc42 and RhoA; (iv) utilization of photoactivated probes
to control the activity of signaling molecules at specific loci such as AJs; (v) mechanical acto-myosin tension
across VE-cadherin adhesion using biosensors. These methods available in Core B will provide the advanced
imaging capabilities needed to address the questions posed. In addition, Core B will provide high-quality
uniformly cultured human and mouse lung microvessel endothelial cells and isolation of lung endothelial cells
from genetic mouse models as needed. The Imaging and Cell Culture Core B will be essential for meeting the
scientific objectives of each Project and the Program as a whole.

## Key facts

- **NIH application ID:** 9970538
- **Project number:** 5P01HL060678-20
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT CHICAGO
- **Principal Investigator:** Yulia A Komarova
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $244,790
- **Award type:** 5
- **Project period:** 2000-03-08 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9970538

## Citation

> US National Institutes of Health, RePORTER application 9970538, Imaging and Cell Culture (5P01HL060678-20). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/9970538. Licensed CC0.

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