# Macromolecular Engineering

> **NIH NIH P01** · UNIVERSITY OF ILLINOIS AT CHICAGO · 2020 · $284,605

## Abstract

Abstract
All Projects propose to employ novel engineered reagents that enable manipulation and interrogation
of individual signaling pathways with precise spatial and temporal control. As described in each Project,
reagents provided by Core C will be used to define the processes that regulate the recovery of the lung
endothelial barrier. Development and optimization of these tools will require significant effort as
described in the Molecular Engineering Core C. The central functions of Core C will be 1) to develop
molecular tools customized for each specific question in individual Projects, 2) to evaluate the new
reagents and establish protocols for their application in primary human and mouse lung microvessel
endothelial cells, and 3) to provide assistance with the application of the tools, troubleshooting, and
analysis of the results. The intent will be to simplify application of new technologies in the Program
Project and allow participants to focus on the proposed questions requiring these reagents. The
specific reagents that will be developed and employed by Core C include: 1) new tools to control
localization and interaction of proteins identified by each Project; 2) reagents for regulation of activity
of selected proteins in living cells, and targeted activation of these proteins in specific complexes and
subcellular locations; and 3) reagents for light-mediated spatio-temporal regulation of the small
GTPases Rac1 and Cdc42 and other GTPases in living cells as needed. Core C will also generate and
optimize reagents for expression of engineered proteins in primary endothelial cells and animal models,
and develop new reagents for manipulation and interrogation of protein interactions and cell signaling
as needed. The tools developed will enable manipulation of individual signaling pathways and
assessment of their roles in the regulation of endothelial barrier recovery and restoration of the integrity
of adherens junctions.

## Key facts

- **NIH application ID:** 9970539
- **Project number:** 5P01HL060678-20
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT CHICAGO
- **Principal Investigator:** ANDREI V KARGINOV
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2020
- **Award amount:** $284,605
- **Award type:** 5
- **Project period:** 2000-03-08 → 2022-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/9970539

## Citation

> US National Institutes of Health, RePORTER application 9970539, Macromolecular Engineering (5P01HL060678-20). Retrieved via AI Analytics 2026-07-12 from https://api.ai-analytics.org/grant/nih/9970539. Licensed CC0.

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